|
Status |
Public on Nov 15, 2011 |
Title |
PPNAD 2 |
Sample type |
RNA |
|
|
Source name |
adrenal tumor
|
Organism |
Homo sapiens |
Characteristics |
ethnicity: Caucasian age: child disease state: primary pigmented nodular adrenocortical disease (PPNAD) tissue: adrenal tumor
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
replicate 1
|
Data processing |
Z score transformation for normalization was performed for each Illumina sample/array as previously described (Cheadle et al. J Mol Diagn, 5:73-81).
|
|
|
Submission date |
Nov 14, 2011 |
Last update date |
Nov 15, 2011 |
Contact name |
Madson Q Almeida |
E-mail(s) |
madsonalmeida@usp.br
|
Phone |
301 451 3768
|
Fax |
301 402 0574
|
Organization name |
NIH
|
Department |
Section on Genetics and Endocrinology / NICHD
|
Lab |
Dr. Stratakis Lab
|
Street address |
10 Center Drive, MSC 1103
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892-1103 |
Country |
USA |
|
|
Platform ID |
GPL6255 |
Series (1) |
GSE33694 |
Activation of cyclic AMP signaling leads to different pathway alterations in lesions of the adrenal cortex caused by germline PRKAR1A defects versus those due to somatic GNAS mutations |
|