|
Status |
Public on Dec 04, 2011 |
Title |
Control replicate 1 |
Sample type |
RNA |
|
|
Source name |
S17 cell control
|
Organism |
Mus musculus |
Characteristics |
cell line: S17 cell type: Stromal cell
|
Treatment protocol |
control
|
Growth protocol |
SC S17 growing in 10-cm dishes were trypsinized in 0.25% trypsin solution and resuspended with MEM containing 2% FBS.
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen Rneasy
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA was prepared according to the instructions of the manufacturer.
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
Description |
Data from control S17 cells SAMPLE 1
|
Data processing |
Rma normalized in R
|
|
|
Submission date |
Nov 15, 2011 |
Last update date |
Dec 04, 2011 |
Contact name |
Ola Larsson |
E-mail(s) |
ola.larsson@ki.se
|
Phone |
+46 (0)8 517 73280
|
Organization name |
Karolinska Institutet
|
Department |
Department of oncology-pathology
|
Street address |
CCK, R8:01
|
City |
Stockholm |
ZIP/Postal code |
171 76 |
Country |
Sweden |
|
|
Platform ID |
GPL7546 |
Series (1) |
GSE33717 |
PDGF-BB modulates hematopoiesis and tumor angiogenesis by inducing erythropoietin production in stromal cells. |
|