|
| Status |
Public on Dec 04, 2011 |
| Title |
Control replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
S17 cell control
|
| Organism |
Mus musculus |
| Characteristics |
cell line: S17
cell type: Stromal cell
|
| Treatment protocol |
control
|
| Growth protocol |
SC S17 growing in 10-cm dishes were trypsinized in 0.25% trypsin solution and resuspended with MEM containing 2% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the instructions of the manufacturer.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Data from control S17 cells
SAMPLE 3
|
| Data processing |
Rma normalized in R
|
| |
|
| Submission date |
Nov 15, 2011 |
| Last update date |
Dec 04, 2011 |
| Contact name |
Ola Larsson |
| E-mail(s) |
ola.larsson@ki.se
|
| Phone |
+46 (0)8 517 73280
|
| Organization name |
Karolinska Institutet
|
| Department |
Department of oncology-pathology
|
| Street address |
CCK, R8:01
|
| City |
Stockholm |
| ZIP/Postal code |
171 76 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL7546 |
| Series (1) |
| GSE33717 |
PDGF-BB modulates hematopoiesis and tumor angiogenesis by inducing erythropoietin production in stromal cells. |
|
