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Sample GSM837299 Query DataSets for GSM837299
Status Public on Nov 19, 2011
Title Wholebrain_NOD_Veh_DD659_1.1
Sample type RNA
 
Source name brain,Wholebrain,NOD,Veh
Organism Mus musculus
Characteristics strain: NOD
treatment: vehicle
part of brain: Wholebrain
body wt (g): 30.9
dob: 2009-08-30
weaned: 2013-09-24
litter size: 9
mouse id: DD0659
time inject: 11:40
time sac: 2:40
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from frozen brains using a Biopulverizer (Biospec). Pulverized samples were placed in tubes containing 1ml Trizol reagent (Invitrogen) and a stainless steel bead. Samples were shaken for 5 minutes on a Tissuelyzer apparatus (Mixer Mill 300, Qiagen) and incubated at room temperature for 5 minutes. We added 200 µl chloroform (Fisher) and then samples were sequentially shaken for 15 seconds on a Tissuelyzer, incubated at room temperature for 3 minutes, and centrifuged at 10,000 rpm for 15 min at 4°C. The supernatant was transferred to a gDNA Eliminator spin column (RNeasy plus mini kit, Qiagen) and processed according to the manufacturer instructions.
Label biotin
Label protocol All samples were processed with the GeneChip WT terminal labeling and hybridization protocol in conjunction with the Ambion WT expression kit according the manufacturers instructions.
 
Hybridization protocol All samples were processed with the GeneChip WT terminal labeling and hybridization protocol in conjunction with the Ambion WT expression kit according the manufacturers instructions.
Scan protocol Affymetrics GeneChip
Data processing Affymetrics Expression Console V1.1
 
Submission date Nov 18, 2011
Last update date Nov 19, 2011
Contact name Vasyl Zhabotynsky
E-mail(s) vasyl@unc.edu
Phone (919) 843-6475
Fax (919) 843-4682
Organization name University of North Carolina at Chapel Hill
Street address 120 Mason Farm Road 5000 D, Genetic Medicine Building
City Chapel Hill
State/province NC
ZIP/Postal code 27599-7264
Country USA
 
Platform ID GPL11533
Series (1)
GSE33822 Transcriptome Atlases Of Mouse Brain Reveals Differential Expression Across Brain Regions And Genetic Backgrounds

Data table header descriptions
ID_REF
VALUE rma gene default signal, log2 transformed

Data table
ID_REF VALUE
10344614 6.362978
10344616 3.047458
10344620 4.579768
10344622 5.927693
10344624 9.24489
10344633 9.223968
10344637 10.62428
10344653 7.564922
10344658 9.481015
10344674 3.538667
10344679 8.044556
10344707 10.48068
10344713 8.951579
10344715 4.356487
10344717 3.680764
10344719 4.573002
10344721 2.948144
10344723 7.021794
10344725 8.243295
10344741 10.20278

Total number of rows: 28310

Table truncated, full table size 494 Kbytes.




Supplementary file Size Download File type/resource
GSM837299.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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