strain: NOD treatment: vehicle part of brain: Hindbrain body wt (g): 32.8 dob: 2009-08-30 weaned: 2013-09-24 litter size: 9 mouse id: DD0656 time inject: 11:20 time sac: 2:20
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated from frozen brains using a Biopulverizer (Biospec). Pulverized samples were placed in tubes containing 1ml Trizol reagent (Invitrogen) and a stainless steel bead. Samples were shaken for 5 minutes on a Tissuelyzer apparatus (Mixer Mill 300, Qiagen) and incubated at room temperature for 5 minutes. We added 200 µl chloroform (Fisher) and then samples were sequentially shaken for 15 seconds on a Tissuelyzer, incubated at room temperature for 3 minutes, and centrifuged at 10,000 rpm for 15 min at 4°C. The supernatant was transferred to a gDNA Eliminator spin column (RNeasy plus mini kit, Qiagen) and processed according to the manufacturer instructions.
Label
biotin
Label protocol
All samples were processed with the GeneChip WT terminal labeling and hybridization protocol in conjunction with the Ambion WT expression kit according the manufacturers instructions.
Hybridization protocol
All samples were processed with the GeneChip WT terminal labeling and hybridization protocol in conjunction with the Ambion WT expression kit according the manufacturers instructions.
