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Sample GSM83864 Query DataSets for GSM83864
Status Public on Jul 31, 2007
Title R6/1 27 week transgenic_2C4C
Sample type RNA
 
Source name Brain hemisphere
Organism Mus musculus
Characteristics Transgenic, brain hemisphere
Extracted molecule total RNA
Extraction protocol Three sex-matched mice from each genotype (R6/1 and wild-type) were sacrificed at 18, 22 and 27 weeks of age. Brain samples were collected, snap frozen in liquid nitrogen and stored at -80°C. Total RNA was extracted from one hemisphere for microarray analysis, as described previously (Morton et al., 2005). Microarray analysis of global gene expression was carried out using 10µg of total RNA and murine genome 430 2.0 Arrays
Label biotin
Label protocol Approximately 10 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description R6/1 transgenic, brain hemisphere
Data processing Affymetrix Microarray Suite version 5.0
 
Submission date Nov 16, 2005
Last update date Aug 28, 2018
Contact name Lesley Jones, Angela Hodges
E-mail(s) jonesl1@cf.ac.uk, Angela.Hodges@iop.kcl.ac.uk
Phone +44 2920 745175
Organization name Cardiff University School of Medicine
Department Psychological Medicine
Street address Heath Park
City Cardiff
ZIP/Postal code CF14 4XN
Country United Kingdom
 
Platform ID GPL1261
Series (1)
GSE3621 R6/1 brain hemisphere time series gene expression
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE detection p-value, p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 63.2 P 0.001794
AFFX-BioB-M_at 68.7 P 0.00006
AFFX-BioB-3_at 56.2 P 0.000857
AFFX-BioC-5_at 212.8 P 0.00011
AFFX-BioC-3_at 254.9 P 0.000044
AFFX-BioDn-5_at 556.9 P 0.000044
AFFX-BioDn-3_at 1168.5 P 0.00006
AFFX-CreX-5_at 3236.6 P 0.000052
AFFX-CreX-3_at 3954.3 P 0.000044
AFFX-DapX-5_at 185.8 P 0.000052
AFFX-DapX-M_at 300.9 P 0.00034
AFFX-DapX-3_at 343.7 P 0.000052
AFFX-LysX-5_at 14.9 P 0.001593
AFFX-LysX-M_at 26.6 P 0.003212
AFFX-LysX-3_at 63.3 P 0.00007
AFFX-PheX-5_at 32.3 P 0.000095
AFFX-PheX-M_at 30.1 P 0.000446
AFFX-PheX-3_at 43.5 P 0.00039
AFFX-ThrX-5_at 61.5 P 0.000258
AFFX-ThrX-M_at 58.3 P 0.000044

Total number of rows: 45101

Table truncated, full table size 1191 Kbytes.




Supplementary file Size Download File type/resource
GSM83864.CEL.gz 5.9 Mb (ftp)(http) CEL

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