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Status
Public on Jul 31, 2007
Title
R6/1 27 week transgenic_75F3
Sample type
RNA
Source name
Brain hemisphere
Organism
Mus musculus Characteristics
Transgenic, brain hemisphere
Extracted molecule
total RNA
Extraction protocol
Three sex-matched mice from each genotype (R6/1 and wild-type) were sacrificed at 18, 22 and 27 weeks of age. Brain samples were collected, snap frozen in liquid nitrogen and stored at -80°C. Total RNA was extracted from one hemisphere for microarray analysis, as described previously (Morton et al., 2005). Microarray analysis of global gene expression was carried out using 10µg of total RNA and murine genome 430 2.0 Arrays
Label
biotin
Label protocol
Approximately 10 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
R6/1 transgenic, brain hemisphere
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Nov 16, 2005
Last update date
Aug 28, 2018
Contact name
Lesley Jones, Angela Hodges
E-mail(s)
jonesl1@cf.ac.uk, Angela.Hodges@iop.kcl.ac.uk Phone
+44 2920 745175
Organization name
Cardiff University School of Medicine
Department
Psychological Medicine
Street address
Heath Park
City
Cardiff
ZIP/Postal code
CF14 4XN
Country
United Kingdom
Platform ID
GPL1261 Series (1)
GSE3621 R6/1 brain hemisphere time series gene expression
Relations Reanalyzed by
GSE119085
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE detection p-value, p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
46.2
P
0.000754
AFFX-BioB-M_at
62.2
P
0.000169
AFFX-BioB-3_at
46.3
P
0.00011
AFFX-BioC-5_at
189.9
P
0.000081
AFFX-BioC-3_at
208.6
P
0.000052
AFFX-BioDn-5_at
477.8
P
0.000044
AFFX-BioDn-3_at
910.6
P
0.000081
AFFX-CreX-5_at
2817.7
P
0.000044
AFFX-CreX-3_at
3218.7
P
0.000044
AFFX-DapX-5_at
112.1
P
0.00006
AFFX-DapX-M_at
229.8
P
0.000446
AFFX-DapX-3_at
238.9
P
0.00007
AFFX-LysX-5_at
12.8
P
0.000389
AFFX-LysX-M_at
32.5
P
0.004998
AFFX-LysX-3_at
52.4
P
0.00007
AFFX-PheX-5_at
17.7
P
0.00034
AFFX-PheX-M_at
20.5
P
0.001796
AFFX-PheX-3_at
36.5
P
0.000972
AFFX-ThrX-5_at
44.4
P
0.001102
AFFX-ThrX-M_at
49.1
P
0.000258
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