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Status
Public on Jul 31, 2007
Title
R6/1 18 week transgenic_C627
Sample type
RNA
Source name
Brain hemisphere
Organism
Mus musculus
Characteristics
Transgenic, brain hemisphere
Extracted molecule
total RNA
Extraction protocol
Three sex-matched mice from each genotype (R6/1 and wild-type) were sacrificed at 18, 22 and 27 weeks of age. Brain samples were collected, snap frozen in liquid nitrogen and stored at -80°C. Total RNA was extracted from one hemisphere for microarray analysis, as described previously (Morton et al., 2005). Microarray analysis of global gene expression was carried out using 10µg of total RNA and murine genome 430 2.0 Arrays
Label
biotin
Label protocol
Approximately 10 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
R6/1 transgenic, brain hemisphere
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Nov 16, 2005
Last update date
Aug 28, 2018
Contact name
Lesley Jones, Angela Hodges
E-mail(s)
jonesl1@cf.ac.uk, Angela.Hodges@iop.kcl.ac.uk
Phone
+44 2920 745175
Organization name
Cardiff University School of Medicine
Department
Psychological Medicine
Street address
Heath Park
City
Cardiff
ZIP/Postal code
CF14 4XN
Country
United Kingdom
Platform ID
GPL1261
Series (1)
GSE3621
R6/1 brain hemisphere time series gene expression
Relations
Reanalyzed by
GSE119085
Data table header descriptions
ID_REF
VALUE
MAS5-calculated Signal intensity
ABS_CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE
detection p-value, p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-BioB-5_at
54.8
P
0.001593
AFFX-BioB-M_at
70.9
P
0.000095
AFFX-BioB-3_at
46.5
P
0.000258
AFFX-BioC-5_at
226.3
P
0.00011
AFFX-BioC-3_at
236.4
P
0.000044
AFFX-BioDn-5_at
574.8
P
0.000044
AFFX-BioDn-3_at
1109.5
P
0.00007
AFFX-CreX-5_at
3241.3
P
0.000052
AFFX-CreX-3_at
4101.2
P
0.000044
AFFX-DapX-5_at
134.7
P
0.000052
AFFX-DapX-M_at
261.7
P
0.000581
AFFX-DapX-3_at
277.7
P
0.000081
AFFX-LysX-5_at
22.3
P
0.001796
AFFX-LysX-M_at
32.5
P
0.030976
AFFX-LysX-3_at
47.1
P
0.000052
AFFX-PheX-5_at
25.8
P
0.000662
AFFX-PheX-M_at
21.6
P
0.000662
AFFX-PheX-3_at
24.4
P
0.000662
AFFX-ThrX-5_at
60.9
P
0.000581
AFFX-ThrX-M_at
51
P
0.00006
Total number of rows: 45101 Table truncated, full table size 1192 Kbytes .Supplementary file
Size
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File type/resource
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CEL