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Sample GSM83879 Query DataSets for GSM83879
Status Public on Jul 31, 2007
Title R6/1 18 week transgenic_BC91
Sample type RNA
 
Source name Brain hemisphere
Organism Mus musculus
Characteristics Transgenic, brain hemisphere
Extracted molecule total RNA
Extraction protocol Three sex-matched mice from each genotype (R6/1 and wild-type) were sacrificed at 18, 22 and 27 weeks of age. Brain samples were collected, snap frozen in liquid nitrogen and stored at -80°C. Total RNA was extracted from one hemisphere for microarray analysis, as described previously (Morton et al., 2005). Microarray analysis of global gene expression was carried out using 10µg of total RNA and murine genome 430 2.0 Arrays
Label biotin
Label protocol Approximately 10 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description R6/1 transgenic, brain hemisphere
Data processing Affymetrix Microarray Suite version 5.0
 
Submission date Nov 16, 2005
Last update date Aug 28, 2018
Contact name Lesley Jones, Angela Hodges
E-mail(s) jonesl1@cf.ac.uk, Angela.Hodges@iop.kcl.ac.uk
Phone +44 2920 745175
Organization name Cardiff University School of Medicine
Department Psychological Medicine
Street address Heath Park
City Cardiff
ZIP/Postal code CF14 4XN
Country United Kingdom
 
Platform ID GPL1261
Series (1)
GSE3621 R6/1 brain hemisphere time series gene expression
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE detection p-value, p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 41.3 P 0.000857
AFFX-BioB-M_at 64.1 P 0.00011
AFFX-BioB-3_at 38.3 P 0.000446
AFFX-BioC-5_at 184.3 P 0.00011
AFFX-BioC-3_at 244 P 0.000052
AFFX-BioDn-5_at 489.4 P 0.000044
AFFX-BioDn-3_at 992.9 P 0.00007
AFFX-CreX-5_at 2904.5 P 0.000052
AFFX-CreX-3_at 3756.9 P 0.000044
AFFX-DapX-5_at 113.6 P 0.000044
AFFX-DapX-M_at 166.5 P 0.000856
AFFX-DapX-3_at 177.3 P 0.000052
AFFX-LysX-5_at 11.9 P 0.000225
AFFX-LysX-M_at 20.2 P 0.030976
AFFX-LysX-3_at 38.7 P 0.000169
AFFX-PheX-5_at 15.9 P 0.000581
AFFX-PheX-M_at 17.1 P 0.011384
AFFX-PheX-3_at 28.1 P 0.004998
AFFX-ThrX-5_at 26.6 P 0.005565
AFFX-ThrX-M_at 39.7 P 0.000169

Total number of rows: 45101

Table truncated, full table size 1190 Kbytes.




Supplementary file Size Download File type/resource
GSM83879.CEL.gz 5.9 Mb (ftp)(http) CEL

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