|
| Status |
Public on Oct 16, 2012 |
| Title |
Th2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
FACS-sorted Th2
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: peripheral blood
cell type: CD4+ CD8- CRTh2+ CCR5- CD45RA- CD25-
|
| Growth protocol |
Cells were nor cultured nor treated in any way, but immediately lysed in TRIzol upon purification from peripheral blood
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells recovered upon sorting were immediately lysed in TRIzol and total RNA was extracted following the manufacture's protocol
|
| Label |
Hy3
|
| Label protocol |
Performed at Exiqon using a miRCURY™ LNA Array Power labeling kit
|
| |
|
| Channel 2 |
| Source name |
total CD4+ lymphocytes
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: CD4+
|
| Growth protocol |
Cells were nor cultured nor treated in any way, but immediately lysed in TRIzol upon purification from peripheral blood
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells recovered upon sorting were immediately lysed in TRIzol and total RNA was extracted following the manufacture's protocol
|
| Label |
Hy5
|
| Label protocol |
Performed at Exiqon using a miRCURY™ LNA Array Power labeling kit
|
| |
|
| |
| Hybridization protocol |
As by miRCURY™ LNA Array Power labeling kit protocol
|
| Scan protocol |
Performed by Exiqon
|
| Description |
Sample 3
common reference (Ch2) was made by pooling together a small amount of total CD4+ cells from all donors.
Cy3 : 1_Exiqon_13973334_S01_Cropped.txt
Cy5 : 0_Exiqon_13973334_S01_Cropped.txt
|
| Data processing |
Performed by Exiqon. Normalisation means background subtraction and normalisation with a global Lowess (LOcally WEighted Scatterplot Smoothing) regression algorithm. Hy3 and Hy5 signals lower than 1.5x of the median signal intensity of the given slide is indicated by 'null'
|
| |
|
| Submission date |
Nov 25, 2011 |
| Last update date |
Oct 16, 2012 |
| Contact name |
Silvia Monticelli |
| E-mail(s) |
silvia.monticelli@irb.usi.ch
|
| Organization name |
Institute for Research in Biomedicine
|
| Street address |
Via Vincenzo Vela 6
|
| City |
Bellinzona |
| ZIP/Postal code |
6500 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL7723 |
| Series (1) |
| GSE33946 |
MicroRNA expression profiling of highly purified CD4+ human T cell subsets |
|
