|
| Status |
Public on Aug 13, 2012 |
| Title |
Lgr5ki_GFPdim_sort_a |
| Sample type |
RNA |
| |
|
| Source name |
FACS sorted epithelial intestinal cells cells from Lgr5-EGFP-ires-CreERT2 mice
|
| Organism |
Mus musculus |
| Characteristics |
lgr5 expression level: low
|
| Growth protocol |
Sorted cells of intestines from Lgr5-EGFP-ires-CreERT2 mice expressing GFP under the control of the Lgr5 promoter were used. RNA was isolated from three independent FACS runs from two different cell populations: one expressing GFP at high levels (GFPbright) and the other expressing GFP at low levels (GFPdim).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol,
|
| Label |
biotin
|
| Label protocol |
RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 100-300ng of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA synthesis Kit (Affymetrix) according to manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
cRNA was hybridized to Affymetrix Mouse HT MG-430 PM plate arrays according to manufacturer’s protocol (Affymetrix).
|
| Scan protocol |
Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol
|
| Description |
ext_151
|
| Data processing |
The expression data extracted from the raw celfiles were normalized with the RMA-sketch algorithm from Affymetrix Power Tools. (All) Data were analyzed using the R2 web application, which is freely available at (http://r2.amc.nl).
|
| |
|
| Submission date |
Nov 25, 2011 |
| Last update date |
Aug 13, 2012 |
| Contact name |
Daniel E. Stange |
| E-mail(s) |
daniel.stange@uniklinikum-dresden.de
|
| Organization name |
University Clinic Dresden
|
| Street address |
Fetscherstr. 74
|
| City |
Dresden |
| ZIP/Postal code |
01307 |
| Country |
Germany |
| |
|
| Platform ID |
GPL11180 |
| Series (2) |
| GSE33948 |
COMPARATIVE TRANSCRIPTOMIC AND PROTEOMIC ANALYSIS OF LGR5+ve STEM CELLS AND THEIR DAUGHTERS (AFFYMETRIX ARRAYS) |
| GSE33949 |
COMPARATIVE TRANSCRIPTOMIC AND PROTEOMIC ANALYSIS OF LGR5+ve STEM CELLS AND THEIR DAUGHTERS |
|
