|
| Status |
Public on Feb 01, 2012 |
| Title |
28-2 low RFP sort 1 3/19 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
28-2 cells, 5% lowest LMP1-mRFP
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: B-cell strain
ebv-status: Positive
expression level: Low LMP1-mRFP
cell line: 28-2
|
| Treatment protocol |
There were no additional treatments applied to these cells.
|
| Growth protocol |
28-2 cells were grown in RPMI-1640 and 10% fetal bovine serum (supplemented with penicillin and streptomycin).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol reagent per manufacturer's protocol
|
| Label |
Hy3
|
| Label protocol |
Conducted by Exiquon per manufacturer's standard protocol.
|
| |
|
| Channel 2 |
| Source name |
28-2 cells, unsorted pool
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: B-cell strain
ebv-status: Positive
expression level: control
cell line: 28-2
|
| Treatment protocol |
There were no additional treatments applied to these cells.
|
| Growth protocol |
28-2 cells were grown in RPMI-1640 and 10% fetal bovine serum (supplemented with penicillin and streptomycin).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol reagent per manufacturer's protocol
|
| Label |
Hy5
|
| Label protocol |
Conducted by Exiquon per manufacturer's standard protocol.
|
| |
|
| |
| Hybridization protocol |
Conducted by Exiquon per manufacturer's standard protocol.
|
| Scan protocol |
Conducted by Exiquon per manufacturer's standard protocol.
|
| Description |
Biological replicate 1 of 3. 5% of 28-2 cells expressing the lowest levels of LMP1-mRFP, isolated by flow cytometry of single cells, harvested immediately after sorting.
|
| Data processing |
LOWESS normalized, background subtraction was Normexp with offset value 10 (Convolution model described by Ritchie ME, Bioinformatics (2007), Vol. 23 no. 20 2007, pages 2700–2707).
|
| |
|
| Submission date |
Nov 28, 2011 |
| Last update date |
Feb 01, 2012 |
| Contact name |
Zachary Pratt |
| E-mail(s) |
zpratt@wisc.edu
|
| Organization name |
University of Wisconsin-Madison
|
| Department |
Oncology
|
| Lab |
Sugden
|
| Street address |
1400 University Avenue
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platform ID |
GPL7723 |
| Series (1) |
| GSE33972 |
Epstein-Barr Virus (EBV)-positive B-cell strain, 28-2: Low v. High latent membrane protein 1 (LMP1)-mRFP expression (miRNA) |
|
