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Sample GSM840148 Query DataSets for GSM840148
Status Public on Aug 31, 2012
Title DeltaPT high d2 c
Sample type RNA
 
Source name Mouse whole lung
Organism Mus musculus
Characteristics strain: BALB/c
gender: female
age: 7 wk
tissue: lung
infection: 30 x 10^6 CFU DeltaPT Bordetella pertussis
time: 2 dpi
Extracted molecule total RNA
Extraction protocol RNA was extracted with RNA STAT-60 reagent (Tel Test, Inc.), followed by DNase I treatment and clean-up with QIAGEN RNeasy mini kit in accordance with the protocol provided with the kit. Quality control was performed with an Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Illumina TotalPrep RNA Amplification Kit from Ambion.
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description deltaPT-treated, high dose, sacrificed 2 dpi, biological replicate 3
Data processing The data were normalized by median method implemented in R package beadarray.
 
Submission date Nov 29, 2011
Last update date Aug 31, 2012
Contact name Nicholas Carbonetti
E-mail(s) ncarbone@umaryland.edu
Organization name University of Maryland Baltimore
Department Microbiology and Immunology
Lab Carbonetti Lab
Street address 685 W. Baltimore St. HSF I-380
City Baltimore
State/province MD
ZIP/Postal code 21201
Country USA
 
Platform ID GPL6885
Series (1)
GSE33995 The effect of pertussis toxin on lung airway gene expression during B. pertussis infection in mice

Data table header descriptions
ID_REF
VALUE median normalized and log2 transformed
Detection PVal

Data table
ID_REF VALUE Detection PVal
ILMN_1212607 7.654460448 0.9787
ILMN_1212612 9.146257199 1
ILMN_1212619 7.70307421 0.98747
ILMN_1212628 7.227541863 0.65789
ILMN_1212632 7.539036052 0.9599
ILMN_1212636 11.79136599 1
ILMN_1212637 11.41945444 1
ILMN_1212645 7.070131681 0.29449
ILMN_1212648 9.016396515 1
ILMN_1212653 9.764857748 1
ILMN_1212672 8.572967415 1
ILMN_1212682 7.415064942 0.8985
ILMN_1212683 7.102088448 0.36717
ILMN_1212685 7.033557066 0.22431
ILMN_1212692 9.247429747 1
ILMN_1212693 9.598396602 1
ILMN_1212695 8.718315162 1
ILMN_1212698 7.078659952 0.31328
ILMN_1212702 15.2407792 1
ILMN_1212703 7.838124171 0.99499

Total number of rows: 25697

Table truncated, full table size 758 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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