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Sample GSM841093 Query DataSets for GSM841093
Status Public on Dec 18, 2011
Title 4hr_C57_GSS_62_blood
Sample type RNA
 
Source name blood
Organism Mus musculus
Characteristics strain: C57BL/6J
exposure: GMA-SS
recovery time: 4h
Treatment protocol Mice were exposed to either air or gas metal arc-stainless steel welding fume for 10 days (3 hours/day) at 40 mg/m3
Extracted molecule total RNA
Extraction protocol Lung RNA isolated from whole lung homogenates using the TRIzol method and cleaned with a Rneasy Mini Kit. Aorta was homogenized with a Qiagen Tissue Lyser and RNA was isolated using the Rneasy fibrous mini kit. Whole blood RNA was isolated using the Mouse RiboPure Blood RNA Isolation Kit and globin RNA was removed using the GLOBINclear kit.
Label biotin
Label protocol Illumina TotalPrep RNA Amplification Kit (Applied Biosystems Inc., Foster City, CA, Catalog #AMIL1791)
 
Hybridization protocol 20 hour hybridization period at 58°C
Scan protocol Illumina BeadStation 500G - BeadArray Reader standard
Description 4560232069_F
replicate 2
Data processing Force positive background correction, variance stabilizing transformation, robust spline normalization
 
Submission date Nov 30, 2011
Last update date Dec 18, 2011
Contact name Patti Christine Zeidler-Erdely
E-mail(s) paz9@cdc.gov
Phone 3042855881
Organization name CDC/NIOSH
Department HELD
Street address 1095 Willowdale Road
City Morgantown
State/province WV
ZIP/Postal code 26505
Country USA
 
Platform ID GPL6885
Series (1)
GSE34056 Type 1 interferon and pattern recognition receptor signaling following particulate matter inhalation

Data table header descriptions
ID_REF
VALUE quantile normalized

Data table
ID_REF VALUE
ILMN_2918977 8.480604926
ILMN_2618329 15.09380206
ILMN_2745741 8.996051786
ILMN_2711892 8.044923642
ILMN_1242531 8.341678926
ILMN_3161404 7.633529836
ILMN_1225633 8.988213437
ILMN_2756696 10.31509056
ILMN_2992517 7.975281414
ILMN_1217625 7.727926209
ILMN_1218866 7.68026346
ILMN_2602185 9.772302911
ILMN_2761487 10.4178564
ILMN_2785605 8.18170028
ILMN_2850839 12.22575909
ILMN_2721178 9.624051359
ILMN_3092673 9.30837268
ILMN_2816356 7.740912867
ILMN_2808939 8.478874158
ILMN_2634564 8.260189944

Total number of rows: 9325

Table truncated, full table size 226 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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