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Sample GSM841100 Query DataSets for GSM841100
Status Public on Dec 18, 2011
Title 28d_C57_GSS_77_blood
Sample type RNA
 
Source name blood
Organism Mus musculus
Characteristics strain: C57BL/6J
exposure: GMA-SS
recovery time: 28d
Treatment protocol Mice were exposed to either air or gas metal arc-stainless steel welding fume for 10 days (3 hours/day) at 40 mg/m3
Extracted molecule total RNA
Extraction protocol Lung RNA isolated from whole lung homogenates using the TRIzol method and cleaned with a Rneasy Mini Kit. Aorta was homogenized with a Qiagen Tissue Lyser and RNA was isolated using the Rneasy fibrous mini kit. Whole blood RNA was isolated using the Mouse RiboPure Blood RNA Isolation Kit and globin RNA was removed using the GLOBINclear kit.
Label biotin
Label protocol Illumina TotalPrep RNA Amplification Kit (Applied Biosystems Inc., Foster City, CA, Catalog #AMIL1791)
 
Hybridization protocol 20 hour hybridization period at 58°C
Scan protocol Illumina BeadStation 500G - BeadArray Reader standard
Description 4560232062_E
replicate 1
Data processing Force positive background correction, variance stabilizing transformation, robust spline normalization
 
Submission date Nov 30, 2011
Last update date Dec 18, 2011
Contact name Patti Christine Zeidler-Erdely
E-mail(s) paz9@cdc.gov
Phone 3042855881
Organization name CDC/NIOSH
Department HELD
Street address 1095 Willowdale Road
City Morgantown
State/province WV
ZIP/Postal code 26505
Country USA
 
Platform ID GPL6885
Series (1)
GSE34056 Type 1 interferon and pattern recognition receptor signaling following particulate matter inhalation

Data table header descriptions
ID_REF
VALUE quantile normalized

Data table
ID_REF VALUE
ILMN_2918977 8.610133435
ILMN_2618329 14.83835683
ILMN_2745741 8.6511947
ILMN_2711892 8.047033206
ILMN_1242531 8.158603629
ILMN_3161404 7.694354124
ILMN_1225633 8.575567072
ILMN_2756696 10.17654199
ILMN_2992517 7.926615374
ILMN_1217625 7.650769551
ILMN_1218866 7.569919178
ILMN_2602185 8.924759946
ILMN_2761487 10.89195241
ILMN_2785605 8.207307434
ILMN_2850839 12.60941443
ILMN_2721178 9.835292284
ILMN_3092673 9.669672199
ILMN_2816356 7.66743406
ILMN_2808939 8.717017105
ILMN_2634564 8.426978784

Total number of rows: 9325

Table truncated, full table size 226 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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