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Status |
Public on Dec 15, 2011 |
Title |
Valproic_acid+Lithium_rep1 |
Sample type |
RNA |
|
|
Source name |
7-day cultured HSPCs with Lithium and Valproic Acid
|
Organism |
Mus musculus |
Characteristics |
strain: C57Bl/6 tissue: Bone Marrow HSPC
|
Growth protocol |
Freshly isolated hematopoietic stem/progenitor cells, defined by L-S+K+ phenotype, were differentiated in a liquid medium supplemented with fetal calf serum, murine stem cell factor and recombinant murine granulocyte/macrophage-colony stimulating factor. The cells were cultured for 7 days with or without 1mM VPA or 5mM LiCl or the combination. At day 7 the cells were collected in RNA lysis buffer (Qiagen) for gene expression studies.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using the RNeasy Mini kit (Qiagen) in accordance with the manufacturer’s protocol.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA was prepared using the Illumina TotalPrep RNA Amplification Kit (Ambion) according to the manufacturer’s specifications starting with 100 ng total RNA.
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|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Data processing |
Quantile normalization was applied on log2-transformed data. Normalized matrix: GeneSpring GX 11.0-computed normalized signal intensity on log2-transformed data. Non-normalized matrix: GenomeStudio-computed raw signal intensity.
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Submission date |
Dec 01, 2011 |
Last update date |
Dec 15, 2011 |
Contact name |
Leonid Bystrykh |
E-mail(s) |
l.bystrykh@umcg.nl
|
Organization name |
University Medical Center Groningen
|
Department |
European Research Institute for the Biology of Ageing - ERIBA
|
Lab |
Ageing Biology and Stem Cells
|
Street address |
A. Deusinglaan 1
|
City |
Groningen |
State/province |
Groningen |
ZIP/Postal code |
9713 AV |
Country |
Netherlands |
|
|
Platform ID |
GPL6885 |
Series (2) |
GSE34088 |
The combination of valproic acid and lithium prevents hematopoietic stem/progenitor cell differentiation. |
GSE35291 |
Expression profiles of 7-day cultured HSPCs in the presence or absence of valproic acid and/or lithium |
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