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Sample GSM842900 Query DataSets for GSM842900
Status Public on May 31, 2015
Title Adipocyte undifferentiated day 0 replicate 1
Sample type RNA
 
Source name T0 adipocytes
Organism Mus musculus
Characteristics time point: day 0
cell type: adipocytes
developmental stage: Adipocyte undifferentiated
treatment: differentiation medium (IBMX), insulin and dexamethasone
cell line: NIH 3T3 L1
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy midi kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with gel electrophoreses.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol using HiScan
Description replicate 1
Data processing The data were normalised using cubic spline normalisation with Illumina Genomestudio 2011.1
 
Submission date Dec 05, 2011
Last update date May 31, 2015
Contact name Martin Irmler
Organization name Helmholtz Zentrum M√ľnchen GmbH
Department Institute of Experimental Genetics
Lab Gene Regulation & Epigenetics
Street address Ingolstaedter Landstrasse 1
City Neuherberg
State/province Bayern
ZIP/Postal code 85764
Country Germany
 
Platform ID GPL6885
Series (1)
GSE34150 Genome-wide expression profiling analysis of a time course of differentiating adipocytes

Data table header descriptions
ID_REF
VALUE cubic spline normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 710.4768 0
ILMN_2721178 156.0732 0
ILMN_3033922 1353.789 0
ILMN_3092673 5464.247 0
ILMN_2816356 22.10869 0.01002506
ILMN_2808939 480.4639 0
ILMN_2634564 458.2508 0
ILMN_2737647 3.104853 0.2443609
ILMN_2734484 300.8546 0
ILMN_2952292 51.86079 0
ILMN_2699078 -2.158906 0.6152882
ILMN_1213681 135.4552 0
ILMN_2735413 0.1637699 0.4160401
ILMN_2735415 -0.8810734 0.5062656
ILMN_2891688 249.2583 0
ILMN_2637698 646.953 0
ILMN_2674228 304.4493 0
ILMN_2601546 2.020127 0.2944862
ILMN_1230831 1.833817 0.3082707
ILMN_2848071 9.76368 0.07769424

Total number of rows: 25697

Table truncated, full table size 737 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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