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Sample GSM842901 Query DataSets for GSM842901
Status Public on May 31, 2015
Title Adipocyte differentiating day 2 replicate 1
Sample type RNA
 
Source name D2 adipocytes
Organism Mus musculus
Characteristics time point: day 2
cell type: adipocytes
developmental stage: Adipocyte differentiating
treatment: differentiation medium (IBMX), insulin and dexamethasone
cell line: NIH 3T3 L1
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy midi kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with gel electrophoreses.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol using HiScan
Description replicate 1
Data processing The data were normalised using cubic spline normalisation with Illumina Genomestudio 2011.1
 
Submission date Dec 05, 2011
Last update date May 31, 2015
Contact name Martin Irmler
Organization name Helmholtz Zentrum M√ľnchen GmbH
Department Institute of Experimental Genetics
Lab Gene Regulation & Epigenetics
Street address Ingolstaedter Landstrasse 1
City Neuherberg
State/province Bayern
ZIP/Postal code 85764
Country Germany
 
Platform ID GPL6885
Series (1)
GSE34150 Genome-wide expression profiling analysis of a time course of differentiating adipocytes

Data table header descriptions
ID_REF
VALUE cubic spline normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 560.7101 0
ILMN_2721178 121.3941 0
ILMN_3033922 1372.298 0
ILMN_3092673 5378.18 0
ILMN_2816356 16.45929 0.01754386
ILMN_2808939 367.598 0
ILMN_2634564 617.45 0
ILMN_2737647 -1.046602 0.4949875
ILMN_2734484 280.7835 0
ILMN_2952292 67.2428 0
ILMN_2699078 -2.074261 0.60401
ILMN_1213681 152.371 0
ILMN_2735413 -0.4356723 0.4486215
ILMN_2735415 3.872581 0.2055138
ILMN_2891688 651.6211 0
ILMN_2637698 557.1812 0
ILMN_2674228 273.7361 0
ILMN_2601546 5.376518 0.1679198
ILMN_1230831 -0.3360873 0.4323308
ILMN_2848071 30.70871 0.005012531

Total number of rows: 25697

Table truncated, full table size 735 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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