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Sample GSM842903 Query DataSets for GSM842903
Status Public on May 31, 2015
Title Adipocyte maturating day 6 replicate 1
Sample type RNA
 
Source name D6 adipocytes
Organism Mus musculus
Characteristics time point: day 6
cell type: adipocytes
developmental stage: Adipocyte maturating
treatment: differentiation medium (IBMX), insulin and dexamethasone, and maturation medium containing insulin
cell line: NIH 3T3 L1
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy midi kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with gel electrophoreses.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol using HiScan
Description replicate 1
Data processing The data were normalised using cubic spline normalisation with Illumina Genomestudio 2011.1
 
Submission date Dec 05, 2011
Last update date May 31, 2015
Contact name Martin Irmler
Organization name Helmholtz Zentrum M√ľnchen GmbH
Department Institute of Experimental Genetics
Lab Gene Regulation & Epigenetics
Street address Ingolstaedter Landstrasse 1
City Neuherberg
State/province Bayern
ZIP/Postal code 85764
Country Germany
 
Platform ID GPL6885
Series (1)
GSE34150 Genome-wide expression profiling analysis of a time course of differentiating adipocytes

Data table header descriptions
ID_REF
VALUE cubic spline normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 526.4958 0
ILMN_2721178 107.2109 0
ILMN_3033922 1582.273 0
ILMN_3092673 5289.993 0
ILMN_2816356 18.93644 0.02506266
ILMN_2808939 447.6865 0
ILMN_2634564 540.0509 0
ILMN_2737647 13.05721 0.05513784
ILMN_2734484 370.242 0
ILMN_2952292 47.82658 0
ILMN_2699078 -1.988343 0.6102757
ILMN_1213681 306.8117 0
ILMN_2735413 5.070168 0.1954887
ILMN_2735415 12.95734 0.05513784
ILMN_2891688 618.8798 0
ILMN_2637698 547.2946 0
ILMN_2674228 223.06 0
ILMN_2601546 6.667228 0.1541353
ILMN_1230831 -2.248647 0.641604
ILMN_2848071 19.83436 0.02005013

Total number of rows: 25697

Table truncated, full table size 740 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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