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Sample GSM842904 Query DataSets for GSM842904
Status Public on May 31, 2015
Title Adipocyte maturating day 8 replicate 1
Sample type RNA
 
Source name D8 adipocytes
Organism Mus musculus
Characteristics time point: day 8
cell type: adipocytes
developmental stage: Adipocyte maturating
treatment: differentiation medium (IBMX), insulin and dexamethasone, and maturation medium containing insulin
cell line: NIH 3T3 L1
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy midi kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with gel electrophoreses.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol using HiScan
Description replicate 1
Data processing The data were normalised using cubic spline normalisation with Illumina Genomestudio 2011.1
 
Submission date Dec 05, 2011
Last update date May 31, 2015
Contact name Martin Irmler
Organization name Helmholtz Zentrum M√ľnchen GmbH
Department Institute of Experimental Genetics
Lab Gene Regulation & Epigenetics
Street address Ingolstaedter Landstrasse 1
City Neuherberg
State/province Bayern
ZIP/Postal code 85764
Country Germany
 
Platform ID GPL6885
Series (1)
GSE34150 Genome-wide expression profiling analysis of a time course of differentiating adipocytes

Data table header descriptions
ID_REF
VALUE cubic spline normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 563.6436 0
ILMN_2721178 144.273 0
ILMN_3033922 1930.308 0
ILMN_3092673 6478.807 0
ILMN_2816356 14.16784 0.04511278
ILMN_2808939 658.4092 0
ILMN_2634564 528.6601 0
ILMN_2737647 8.43368 0.0952381
ILMN_2734484 357.4056 0
ILMN_2952292 34.70784 0.001253133
ILMN_2699078 0.565672 0.3734336
ILMN_1213681 306.6847 0
ILMN_2735413 8.44982 0.09398496
ILMN_2735415 13.29452 0.05012531
ILMN_2891688 638.1759 0
ILMN_2637698 489.1173 0
ILMN_2674228 280.9768 0
ILMN_2601546 5.106447 0.1779449
ILMN_1230831 -2.654972 0.6654136
ILMN_2848071 21.21379 0.01002506

Total number of rows: 25697

Table truncated, full table size 737 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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