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Sample GSM842905 Query DataSets for GSM842905
Status Public on May 31, 2015
Title Adipocyte maturating day 10 replicate 1
Sample type RNA
Source name D10 adipocytes
Organism Mus musculus
Characteristics time point: day 10
cell type: adipocytes
developmental stage: Adipocyte maturating
treatment: differentiation medium (IBMX), insulin and dexamethasone, and maturation medium containing insulin
cell line: NIH 3T3 L1
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy midi kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with gel electrophoreses.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol using HiScan
Description replicate 1
Data processing The data were normalised using cubic spline normalisation with Illumina Genomestudio 2011.1
Submission date Dec 05, 2011
Last update date May 31, 2015
Contact name Martin Irmler
Organization name Helmholtz Zentrum M√ľnchen GmbH
Department Institute of Experimental Genetics
Lab Gene Regulation & Epigenetics
Street address Ingolstaedter Landstrasse 1
City Neuherberg
State/province Bayern
ZIP/Postal code 85764
Country Germany
Platform ID GPL6885
Series (1)
GSE34150 Genome-wide expression profiling analysis of a time course of differentiating adipocytes

Data table header descriptions
VALUE cubic spline normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 635.9869 0
ILMN_2721178 124.1849 0
ILMN_3033922 2221.097 0
ILMN_3092673 7087.183 0
ILMN_2816356 15.87311 0.02255639
ILMN_2808939 877.477 0
ILMN_2634564 460.9087 0
ILMN_2737647 -3.849884 0.7769424
ILMN_2734484 432.3885 0
ILMN_2952292 39.12426 0
ILMN_2699078 -1.11325 0.5125313
ILMN_1213681 255.6891 0
ILMN_2735413 3.164383 0.235589
ILMN_2735415 1.464794 0.3433584
ILMN_2891688 635.1791 0
ILMN_2637698 486.3869 0
ILMN_2674228 264.5497 0
ILMN_2601546 16.81274 0.02005013
ILMN_1230831 2.375186 0.2882206
ILMN_2848071 14.08959 0.03634085

Total number of rows: 25697

Table truncated, full table size 738 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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