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Sample GSM842909 Query DataSets for GSM842909
Status Public on May 31, 2015
Title Adipocyte differentiating day 2 replicate 2
Sample type RNA
Source name D2 adipocytes
Organism Mus musculus
Characteristics time point: day 2
cell type: adipocytes
developmental stage: Adipocyte differentiating
treatment: differentiation medium (IBMX), insulin and dexamethasone
cell line: NIH 3T3 L1
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy midi kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with gel electrophoreses.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol using HiScan
Description replicate 2
Data processing The data were normalised using cubic spline normalisation with Illumina Genomestudio 2011.1
Submission date Dec 05, 2011
Last update date May 31, 2015
Contact name Martin Irmler
Organization name Helmholtz Zentrum M√ľnchen GmbH
Department Institute of Experimental Genetics
Lab Gene Regulation & Epigenetics
Street address Ingolstaedter Landstrasse 1
City Neuherberg
State/province Bayern
ZIP/Postal code 85764
Country Germany
Platform ID GPL6885
Series (1)
GSE34150 Genome-wide expression profiling analysis of a time course of differentiating adipocytes

Data table header descriptions
VALUE cubic spline normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 578.4343 0
ILMN_2721178 107.4722 0
ILMN_3033922 1318.332 0
ILMN_3092673 5437.06 0
ILMN_2816356 22.53902 0.01378446
ILMN_2808939 383.3691 0
ILMN_2634564 547.1428 0
ILMN_2737647 10.56919 0.08270676
ILMN_2734484 565.5289 0
ILMN_2952292 98.32157 0
ILMN_2699078 -1.103146 0.5137845
ILMN_1213681 160.4087 0
ILMN_2735413 -3.309669 0.7318296
ILMN_2735415 5.268594 0.1766917
ILMN_2891688 720.3445 0
ILMN_2637698 511.51 0
ILMN_2674228 259.9077 0
ILMN_2601546 11.33869 0.0689223
ILMN_1230831 2.543572 0.2844611
ILMN_2848071 19.24 0.01879699

Total number of rows: 25697

Table truncated, full table size 740 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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