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Sample GSM842910 Query DataSets for GSM842910
Status Public on May 31, 2015
Title Adipocyte differentiating day 4 replicate 2
Sample type RNA
 
Source name D4 adipocytes
Organism Mus musculus
Characteristics time point: day 4
cell type: adipocytes
developmental stage: Adipocyte differentiating
treatment: differentiation medium (IBMX), insulin and dexamethasone
cell line: NIH 3T3 L1
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy midi kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with gel electrophoreses.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol using HiScan
Description replicate 2
Data processing The data were normalised using cubic spline normalisation with Illumina Genomestudio 2011.1
 
Submission date Dec 05, 2011
Last update date May 31, 2015
Contact name Martin Irmler
Organization name Helmholtz Zentrum M√ľnchen GmbH
Department Institute of Experimental Genetics
Lab Gene Regulation & Epigenetics
Street address Ingolstaedter Landstrasse 1
City Neuherberg
State/province Bayern
ZIP/Postal code 85764
Country Germany
 
Platform ID GPL6885
Series (1)
GSE34150 Genome-wide expression profiling analysis of a time course of differentiating adipocytes

Data table header descriptions
ID_REF
VALUE cubic spline normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 553.2229 0
ILMN_2721178 103.318 0
ILMN_3033922 1613.31 0
ILMN_3092673 5137.701 0
ILMN_2816356 7.265097 0.1390978
ILMN_2808939 475.4297 0
ILMN_2634564 554.923 0
ILMN_2737647 6.451879 0.1541353
ILMN_2734484 791.9952 0
ILMN_2952292 54.07888 0
ILMN_2699078 0.2570446 0.4047619
ILMN_1213681 208.6038 0
ILMN_2735413 -0.001197081 0.414787
ILMN_2735415 0.9661863 0.3659148
ILMN_2891688 1011.342 0
ILMN_2637698 445.2788 0
ILMN_2674228 223.4594 0
ILMN_2601546 4.125672 0.2205514
ILMN_1230831 -0.1565285 0.424812
ILMN_2848071 11.29189 0.07769424

Total number of rows: 25697

Table truncated, full table size 744 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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