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Sample GSM842912 Query DataSets for GSM842912
Status Public on May 31, 2015
Title Adipocyte maturating day 8 replicate 2
Sample type RNA
 
Source name D8 adipocytes
Organism Mus musculus
Characteristics time point: day 8
cell type: adipocytes
developmental stage: Adipocyte maturating
treatment: differentiation medium (IBMX), insulin and dexamethasone, and maturation medium containing insulin
cell line: NIH 3T3 L1
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy midi kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with gel electrophoreses.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol using HiScan
Description replicate 2
Data processing The data were normalised using cubic spline normalisation with Illumina Genomestudio 2011.1
 
Submission date Dec 05, 2011
Last update date May 31, 2015
Contact name Martin Irmler
Organization name Helmholtz Zentrum M√ľnchen GmbH
Department Institute of Experimental Genetics
Lab Gene Regulation & Epigenetics
Street address Ingolstaedter Landstrasse 1
City Neuherberg
State/province Bayern
ZIP/Postal code 85764
Country Germany
 
Platform ID GPL6885
Series (1)
GSE34150 Genome-wide expression profiling analysis of a time course of differentiating adipocytes

Data table header descriptions
ID_REF
VALUE cubic spline normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 542.8934 0
ILMN_2721178 98.96171 0
ILMN_3033922 1914.099 0
ILMN_3092673 6570.968 0
ILMN_2816356 19.27264 0.007518797
ILMN_2808939 606.1127 0
ILMN_2634564 495.8111 0
ILMN_2737647 6.365113 0.1353384
ILMN_2734484 682.7175 0
ILMN_2952292 30.91131 0.002506266
ILMN_2699078 -5.230452 0.8934837
ILMN_1213681 241.8992 0
ILMN_2735413 1.623065 0.3082707
ILMN_2735415 8.318622 0.09398496
ILMN_2891688 777.1669 0
ILMN_2637698 542.3491 0
ILMN_2674228 210.0483 0
ILMN_2601546 3.650702 0.2130326
ILMN_1230831 1.536304 0.3107769
ILMN_2848071 8.5875 0.08897243

Total number of rows: 25697

Table truncated, full table size 741 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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