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Sample GSM842913 Query DataSets for GSM842913
Status Public on May 31, 2015
Title Adipocyte maturating day 10 replicate 2
Sample type RNA
Source name D10 adipocytes
Organism Mus musculus
Characteristics time point: day 10
cell type: adipocytes
developmental stage: Adipocyte maturating
treatment: differentiation medium (IBMX), insulin and dexamethasone, and maturation medium containing insulin
cell line: NIH 3T3 L1
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy midi kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with gel electrophoreses.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol using HiScan
Description replicate 2
Data processing The data were normalised using cubic spline normalisation with Illumina Genomestudio 2011.1
Submission date Dec 05, 2011
Last update date May 31, 2015
Contact name Martin Irmler
Organization name Helmholtz Zentrum M√ľnchen GmbH
Department Institute of Experimental Genetics
Lab Gene Regulation & Epigenetics
Street address Ingolstaedter Landstrasse 1
City Neuherberg
State/province Bayern
ZIP/Postal code 85764
Country Germany
Platform ID GPL6885
Series (1)
GSE34150 Genome-wide expression profiling analysis of a time course of differentiating adipocytes

Data table header descriptions
VALUE cubic spline normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 496.6506 0
ILMN_2721178 74.98417 0
ILMN_3033922 1786.435 0
ILMN_3092673 6304.692 0
ILMN_2816356 11.68851 0.04385965
ILMN_2808939 833.3861 0
ILMN_2634564 529.3398 0
ILMN_2737647 2.141413 0.273183
ILMN_2734484 598.0845 0
ILMN_2952292 38.35928 0.001253133
ILMN_2699078 -6.629027 0.9661654
ILMN_1213681 195.8681 0
ILMN_2735413 -0.8359151 0.4711779
ILMN_2735415 1.635057 0.2969925
ILMN_2891688 700.5745 0
ILMN_2637698 508.5963 0
ILMN_2674228 178.5405 0
ILMN_2601546 11.24161 0.04511278
ILMN_1230831 -0.4339732 0.4423559
ILMN_2848071 5.86546 0.1240602

Total number of rows: 25697

Table truncated, full table size 746 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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