|
| Status |
Public on May 31, 2015 |
| Title |
Adipocyte maturating day 18 replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
D18 adipocytes
|
| Organism |
Mus musculus |
| Characteristics |
time point: day 18
cell type: adipocytes
developmental stage: Adipocyte maturating
treatment: differentiation medium (IBMX), insulin and dexamethasone, and maturation medium containing insulin
cell line: NIH 3T3 L1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy midi kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with gel electrophoreses.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol using HiScan
|
| Description |
replicate 2
|
| Data processing |
The data were normalised using cubic spline normalisation with Illumina Genomestudio 2011.1
|
| |
|
| Submission date |
Dec 05, 2011 |
| Last update date |
May 31, 2015 |
| Contact name |
Martin Irmler |
| Organization name |
Helmholtz Zentrum München GmbH
|
| Department |
Institute of Experimental Genetics
|
| Lab |
Gene Regulation & Epigenetics
|
| Street address |
Ingolstaedter Landstrasse 1
|
| City |
Neuherberg |
| State/province |
Bayern |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE34150 |
Genome-wide expression profiling analysis of a time course of differentiating adipocytes |
|
