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Sample GSM842919 Query DataSets for GSM842919
Status Public on May 31, 2015
Title Adipocyte maturating day 6 replicate 3
Sample type RNA
Source name D6 adipocytes
Organism Mus musculus
Characteristics time point: day 6
cell type: adipocytes
developmental stage: Adipocyte maturating
treatment: differentiation medium (IBMX), insulin and dexamethasone, and maturation medium containing insulin
cell line: NIH 3T3 L1
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy midi kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with gel electrophoreses.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol using HiScan
Description replicate 3
Data processing The data were normalised using cubic spline normalisation with Illumina Genomestudio 2011.1
Submission date Dec 05, 2011
Last update date May 31, 2015
Contact name Martin Irmler
Organization name Helmholtz Zentrum M√ľnchen GmbH
Department Institute of Experimental Genetics
Lab Gene Regulation & Epigenetics
Street address Ingolstaedter Landstrasse 1
City Neuherberg
State/province Bayern
ZIP/Postal code 85764
Country Germany
Platform ID GPL6885
Series (1)
GSE34150 Genome-wide expression profiling analysis of a time course of differentiating adipocytes

Data table header descriptions
VALUE cubic spline normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 499.2099 0
ILMN_2721178 107.8193 0
ILMN_3033922 1690.687 0
ILMN_3092673 5604.418 0
ILMN_2816356 8.367715 0.09273183
ILMN_2808939 443.8487 0
ILMN_2634564 558.4977 0
ILMN_2737647 14.61804 0.04135338
ILMN_2734484 631.7706 0
ILMN_2952292 39.20819 0.002506266
ILMN_2699078 -3.829802 0.8057644
ILMN_1213681 304.602 0
ILMN_2735413 5.454069 0.1629073
ILMN_2735415 7.561004 0.1077694
ILMN_2891688 744.9771 0
ILMN_2637698 449.6601 0
ILMN_2674228 258.9572 0
ILMN_2601546 10.94933 0.07393484
ILMN_1230831 -1.463029 0.5463659
ILMN_2848071 22.77937 0.00877193

Total number of rows: 25697

Table truncated, full table size 742 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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