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Sample GSM842920 Query DataSets for GSM842920
Status Public on May 31, 2015
Title Adipocyte maturating day 8 replicate 3
Sample type RNA
Source name D8 adipocytes
Organism Mus musculus
Characteristics time point: day 8
cell type: adipocytes
developmental stage: Adipocyte maturating
treatment: differentiation medium (IBMX), insulin and dexamethasone, and maturation medium containing insulin
cell line: NIH 3T3 L1
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy midi kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with gel electrophoreses.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol using HiScan
Description replicate 3
Data processing The data were normalised using cubic spline normalisation with Illumina Genomestudio 2011.1
Submission date Dec 05, 2011
Last update date May 31, 2015
Contact name Martin Irmler
Organization name Helmholtz Zentrum M√ľnchen GmbH
Department Institute of Experimental Genetics
Lab Gene Regulation & Epigenetics
Street address Ingolstaedter Landstrasse 1
City Neuherberg
State/province Bayern
ZIP/Postal code 85764
Country Germany
Platform ID GPL6885
Series (1)
GSE34150 Genome-wide expression profiling analysis of a time course of differentiating adipocytes

Data table header descriptions
VALUE cubic spline normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 599.6031 0
ILMN_2721178 126.475 0
ILMN_3033922 2102.573 0
ILMN_3092673 6776.416 0
ILMN_2816356 13.57618 0.03508772
ILMN_2808939 771.2517 0
ILMN_2634564 593.7319 0
ILMN_2737647 12.86868 0.03884712
ILMN_2734484 542.918 0
ILMN_2952292 42.49915 0
ILMN_2699078 -1.351244 0.547619
ILMN_1213681 278.9211 0
ILMN_2735413 2.351582 0.2932331
ILMN_2735415 9.87619 0.0689223
ILMN_2891688 707.0457 0
ILMN_2637698 433.6105 0
ILMN_2674228 244.663 0
ILMN_2601546 6.268388 0.1441103
ILMN_1230831 3.013792 0.2556391
ILMN_2848071 16.93678 0.01879699

Total number of rows: 25697

Table truncated, full table size 737 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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