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Sample GSM842923 Query DataSets for GSM842923
Status Public on May 31, 2015
Title Adipocyte maturating day 18 replicate 3
Sample type RNA
 
Source name D18 adipocytes
Organism Mus musculus
Characteristics time point: day 18
cell type: adipocytes
developmental stage: Adipocyte maturating
treatment: differentiation medium (IBMX), insulin and dexamethasone, and maturation medium containing insulin
cell line: NIH 3T3 L1
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy midi kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with gel electrophoreses.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol using HiScan
Description replicate 3
Data processing The data were normalised using cubic spline normalisation with Illumina Genomestudio 2011.1
 
Submission date Dec 05, 2011
Last update date May 31, 2015
Contact name Martin Irmler
Organization name Helmholtz Zentrum M√ľnchen GmbH
Department Institute of Experimental Genetics
Lab Gene Regulation & Epigenetics
Street address Ingolstaedter Landstrasse 1
City Neuherberg
State/province Bayern
ZIP/Postal code 85764
Country Germany
 
Platform ID GPL6885
Series (1)
GSE34150 Genome-wide expression profiling analysis of a time course of differentiating adipocytes

Data table header descriptions
ID_REF
VALUE cubic spline normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 633.2521 0
ILMN_2721178 153.3086 0
ILMN_3033922 2544.136 0
ILMN_3092673 7374.15 0
ILMN_2816356 9.257778 0.0726817
ILMN_2808939 1130.718 0
ILMN_2634564 481.4071 0
ILMN_2737647 7.081005 0.1077694
ILMN_2734484 659.6249 0
ILMN_2952292 45.64173 0
ILMN_2699078 -2.578261 0.660401
ILMN_1213681 238.7131 0
ILMN_2735413 0.5710093 0.3822055
ILMN_2735415 6.356135 0.1203008
ILMN_2891688 738.9034 0
ILMN_2637698 523.6398 0
ILMN_2674228 215.5364 0
ILMN_2601546 3.567068 0.216792
ILMN_1230831 -0.4981562 0.4536341
ILMN_2848071 12.8637 0.04511278

Total number of rows: 25697

Table truncated, full table size 738 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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