|
Status |
Public on Feb 22, 2012 |
Title |
MLL1_mouse_ChIPSeq |
Sample type |
SRA |
|
|
Source name |
MLL-AF9 transfromed blasts
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 cell origin: 5-FU treated BM cells transduced with Flag-tagged MLL-AF9 chip antibody: anti-MLL1
|
Treatment protocol |
Cells were subjected to ChIP-Seq using an antibody for MLL1
|
Growth protocol |
Murine bone marrow cells were transformed with Flag-tagged MLL-AF9 , harvested after two rounds of replating.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
ChIP DNA libraries were made following Illumina ChIP-seq library preparation kit
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina Genome Analyzer IIx |
|
|
Description |
Chromatin IP using anti-MLL1 antibody
|
Data processing |
Sequence reads were aligned to mouse genome assembly mm8 using Bowtie. ChIP-Seq signal was quantified as total number of reads per million in the region of interest. An empirical background distribution model of reads was constructed to find the significance level of signal in a given region.
|
|
|
Submission date |
Dec 08, 2011 |
Last update date |
May 15, 2019 |
Contact name |
Amit Sinha |
E-mail(s) |
amit.sinha@childrens.harvard.edu
|
Phone |
617-582-7579
|
Organization name |
Dana-Farber Cancer Institute
|
Department |
Pediatric Oncology
|
Lab |
Armstrong Lab
|
Street address |
44 Binney St
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02135 |
Country |
USA |
|
|
Platform ID |
GPL11002 |
Series (1) |
GSE34261 |
ChipSeq of MLL1 in mouse leukemic blast cells |
|
Relations |
SRA |
SRX110650 |
BioSample |
SAMN00764093 |