|
Status |
Public on Dec 10, 2011 |
Title |
nodex_1 |
Sample type |
RNA |
|
|
Source name |
cultured human airway smooth muscle, no treatment, replicate 1
|
Organism |
Homo sapiens |
Characteristics |
sample type: no history of asthma tissue: smooth muscle cells treatment: none
|
Treatment protocol |
Cells were treated for 4 hours or 24 hours with 1 micromnolar dexamethasone dissolved in ethanol
|
Growth protocol |
Primary cultured human airway smoothmuscle were grown in Hams/F12 with 10% FBS
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using Trizol frolloed by purification with Invitrogne midilink columns
|
Label |
Cy3
|
Label protocol |
RNA was labeled with Cy3-CTP using the Agilent low RNA input fluorescent linear amplification kits following the manufacturers protocol
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|
|
Hybridization protocol |
Labeled cRNA was assessed using the Nandrop ND-100 (Nanodrop Technologies, Inc., Wilmington DE), and equal amounts of Cy3 labeled target were hybridized to Agilent whole mouse genome 4x44K Ink-jet arrays for 14 hours
|
Scan protocol |
Arrays were scanned using the Agilent microarray scanner
|
Description |
baseline human airway smooth muscle gene expression
|
Data processing |
raw signal intensities were extracted with Feature Extraction v9.1 software
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|
|
Submission date |
Dec 09, 2011 |
Last update date |
Dec 10, 2011 |
Contact name |
Anthony Gerber |
E-mail(s) |
tgerber@alum.mit.edu
|
Phone |
3032702783
|
Organization name |
National Jewish Health
|
Department |
Medicine
|
Street address |
1400 Jackson St
|
City |
Denver |
State/province |
CO |
ZIP/Postal code |
80238 |
Country |
USA |
|
|
Platform ID |
GPL6480 |
Series (1) |
GSE34313 |
Airway smooth muscle reponse to dexamathasone at 4 and 24 hours |
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