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Sample GSM848134 Query DataSets for GSM848134
Status Public on Jul 11, 2012
Title Muscle WT Adult 07272
Sample type RNA
 
Source name Tibialis Anterior Muscle, Wildtype, Adult
Organism Mus musculus
Characteristics strain: wt
genetic background: 129/SVE
tissue: tibialis anterior
age: 7-9 wk
Extracted molecule total RNA
Extraction protocol Rneasy Kit (Qaigen) extraction of total RNA was performed
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2-5 ug total RNA
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip Mouse Genome 430A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS5) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Dec 12, 2011
Last update date Jul 11, 2012
Contact name Gretchen Meyer
Organization name University of California San Diego
Department Bioengineering
Street address 9500 Gilman Dr
City La Jolla
State/province CA
ZIP/Postal code 92122
Country USA
 
Platform ID GPL8321
Series (1)
GSE34388 Transcriptional Alterations in Skeletal Muscle Following Desmin Deletion

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 401.0473
AFFX-BioB-M_at 473.75095
AFFX-BioB-3_at 353.8901
AFFX-BioC-5_at 1078.681
AFFX-BioC-3_at 1489.2422
AFFX-BioDn-5_at 3647.515
AFFX-BioDn-3_at 6172.731
AFFX-CreX-5_at 18165.695
AFFX-CreX-3_at 18849.012
AFFX-DapX-5_at 2.8299634
AFFX-DapX-M_at 2.6885645
AFFX-DapX-3_at 7.257926
AFFX-LysX-5_at 12.89131
AFFX-LysX-M_at 23.685936
AFFX-LysX-3_at 23.478853
AFFX-PheX-5_at 1.0489378
AFFX-PheX-M_at 1
AFFX-PheX-3_at 18.068619
AFFX-ThrX-5_at 1.5660298
AFFX-ThrX-M_at 16.405495

Total number of rows: 22690

Table truncated, full table size 470 Kbytes.




Supplementary file Size Download File type/resource
GSM848134_07272_YWT_C.as.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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