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Sample GSM848139 Query DataSets for GSM848139
Status Public on Jul 11, 2012
Title Muscle WT Aged 12171
Sample type RNA
 
Source name Tibialis Anterior Muscle, Wildtype, Aged
Organism Mus musculus
Characteristics strain: wt
genetic background: 129/SVE
tissue: tibialis anterior
age: 12-14 mo
Extracted molecule total RNA
Extraction protocol Rneasy Kit (Qaigen) extraction of total RNA was performed
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2-5 ug total RNA
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip Mouse Genome 430A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS5) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Dec 12, 2011
Last update date Jul 11, 2012
Contact name Gretchen Meyer
Organization name University of California San Diego
Department Bioengineering
Street address 9500 Gilman Dr
City La Jolla
State/province CA
ZIP/Postal code 92122
Country USA
 
Platform ID GPL8321
Series (1)
GSE34388 Transcriptional Alterations in Skeletal Muscle Following Desmin Deletion

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 370.83206
AFFX-BioB-M_at 596.58636
AFFX-BioB-3_at 390.49374
AFFX-BioC-5_at 1097.353
AFFX-BioC-3_at 1429.331
AFFX-BioDn-5_at 3277.7458
AFFX-BioDn-3_at 4589.7847
AFFX-CreX-5_at 14453.042
AFFX-CreX-3_at 14784.6045
AFFX-DapX-5_at 177.74893
AFFX-DapX-M_at 665.8329
AFFX-DapX-3_at 1162.5148
AFFX-LysX-5_at 69.62435
AFFX-LysX-M_at 95.30847
AFFX-LysX-3_at 189.25699
AFFX-PheX-5_at 68.42568
AFFX-PheX-M_at 78.57671
AFFX-PheX-3_at 122.28357
AFFX-ThrX-5_at 30.79623
AFFX-ThrX-M_at 84.40406

Total number of rows: 22690

Table truncated, full table size 469 Kbytes.




Supplementary file Size Download File type/resource
GSM848139_12171_OWT_C.as.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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