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Sample GSM848140 Query DataSets for GSM848140
Status Public on Jul 11, 2012
Title Muscle WT Aged 12172
Sample type RNA
 
Source name Tibialis Anterior Muscle, Wildtype, Aged
Organism Mus musculus
Characteristics strain: wt
genetic background: 129/SVE
tissue: tibialis anterior
age: 12-14 mo
Extracted molecule total RNA
Extraction protocol Rneasy Kit (Qaigen) extraction of total RNA was performed
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2-5 ug total RNA
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip Mouse Genome 430A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS5) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Dec 12, 2011
Last update date Jul 11, 2012
Contact name Gretchen Meyer
Organization name University of California San Diego
Department Bioengineering
Street address 9500 Gilman Dr
City La Jolla
State/province CA
ZIP/Postal code 92122
Country USA
 
Platform ID GPL8321
Series (1)
GSE34388 Transcriptional Alterations in Skeletal Muscle Following Desmin Deletion

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 490.54578
AFFX-BioB-M_at 640.74054
AFFX-BioB-3_at 448.67114
AFFX-BioC-5_at 1268.8507
AFFX-BioC-3_at 1436.3145
AFFX-BioDn-5_at 3584.4253
AFFX-BioDn-3_at 4370.4624
AFFX-CreX-5_at 16979.873
AFFX-CreX-3_at 17819.74
AFFX-DapX-5_at 240.83083
AFFX-DapX-M_at 917.96185
AFFX-DapX-3_at 1506.2773
AFFX-LysX-5_at 81.82305
AFFX-LysX-M_at 102.48841
AFFX-LysX-3_at 214.43912
AFFX-PheX-5_at 75.98479
AFFX-PheX-M_at 97.99657
AFFX-PheX-3_at 192.57553
AFFX-ThrX-5_at 42.149113
AFFX-ThrX-M_at 67.335464

Total number of rows: 22690

Table truncated, full table size 470 Kbytes.




Supplementary file Size Download File type/resource
GSM848140_12172_OWT_C.as.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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