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Sample GSM848147 Query DataSets for GSM848147
Status Public on Jul 11, 2012
Title Muscle KO Aged 1106
Sample type RNA
 
Source name Tibialis Anterior Muscle, Desmin Knockout, Aged
Organism Mus musculus
Characteristics strain: Des(-/-)/129/SVE
genetic background: 129/SVE
tissue: tibialis anterior
age: 12-14 mo
Extracted molecule total RNA
Extraction protocol Rneasy Kit (Qaigen) extraction of total RNA was performed
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2-5 ug total RNA
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip Mouse Genome 430A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS5) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Dec 12, 2011
Last update date Jul 11, 2012
Contact name Gretchen Meyer
Organization name University of California San Diego
Department Bioengineering
Street address 9500 Gilman Dr
City La Jolla
State/province CA
ZIP/Postal code 92122
Country USA
 
Platform ID GPL8321
Series (1)
GSE34388 Transcriptional Alterations in Skeletal Muscle Following Desmin Deletion

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 399.51547
AFFX-BioB-M_at 679.3387
AFFX-BioB-3_at 415.76062
AFFX-BioC-5_at 1140.0874
AFFX-BioC-3_at 1657.9559
AFFX-BioDn-5_at 2548.3945
AFFX-BioDn-3_at 4273.5796
AFFX-CreX-5_at 12331.834
AFFX-CreX-3_at 16745.357
AFFX-DapX-5_at 2.9715204
AFFX-DapX-M_at 2.0307274
AFFX-DapX-3_at 23.045767
AFFX-LysX-5_at 2.0667226
AFFX-LysX-M_at 5.2061605
AFFX-LysX-3_at 29.0598
AFFX-PheX-5_at 3.4437973
AFFX-PheX-M_at 8.318304
AFFX-PheX-3_at 37.716763
AFFX-ThrX-5_at 5.7962136
AFFX-ThrX-M_at 7.9059286

Total number of rows: 22690

Table truncated, full table size 471 Kbytes.




Supplementary file Size Download File type/resource
GSM848147_1106_OKO_C.as.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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