|
| Status |
Public on Apr 04, 2006 |
| Title |
DCN_P3_C |
| Sample type |
RNA |
| |
|
| Source name |
anterior interpositus nucleus-centered deep cerebellar nuclei, trained with paired paradigm of delayed-type eyeblink conditioning for 3 days
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6
Gender: Male
Age: 11-18wks
Tissue: Anterior interpositus nucleus-centered deep cerebellar nuclei
|
| Biomaterial provider |
Clea Japan, Tokyo, Japan
|
| Treatment protocol |
Mice received a surgery for implanting four Teflon-coated stainless-steel wires in their left eyelid and a headstage on their head. After 3days’ recovery, they were trained with paired paradigm of conditioned stimulus (CS) and unconditioned stimulus (US) for 3days: A 352-ms tone CS (1kHz, 83~85dB) was delivered first and a 100ms periorbital shock US (100kHz square pluses) were delivered with 252ms after the onset of CS, and they co-terminated. After the last trial was given, anterior interpositus was immediately sampled in 10 to 30 min from the sacrifice.
|
| Growth protocol |
Mice were individually housed in a ventilated plastic cage, which was placed in a temperature and humidity-controlled room with 12-h light/dark cycle (light on from 8:00 AM to 8:00 PM). All animals were fed laboratory chow and water ad libitum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
10~15 anterior interpositus-centered deep cerebellar nuclei ipsilateral to the eye implanted with four wires were pooled. Total RNA was extracted using an RNeasy Mini Kit (QIAGEN, Hilden, Germany) and QIAshredder (QIAGEN) according to the manufacturer’s instructions
|
| Label |
Biotin
|
| Label protocol |
18ug Biotin-labeled cRNA was synthesized with BioArray HighYield RNA Transcript Labeling Kit (ENZO Diagnostics Inc., Farmingdale, NY) according to the manufacturer’s instructions
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Anterior interpositus nucleus-centered deep cerebellar nuclei were sampled using syringe needles
|
| Data processing |
Affy version 1.3.25. with default parameters.
|
| |
|
| Submission date |
Nov 23, 2005 |
| Last update date |
Mar 29, 2006 |
| Contact name |
Jin-Sung Park |
| E-mail(s) |
parkjs@brain.riken.jp
|
| Phone |
+81-48-462-9724
|
| Organization name |
RIKEN
|
| Department |
BSI
|
| Lab |
Lab. for Behavioral Genetics
|
| Street address |
2-1 Hirosawa
|
| City |
Wako-shi |
| State/province |
Saitama |
| ZIP/Postal code |
351-0198 |
| Country |
Japan |
| |
|
| Platform ID |
GPL83 |
| Series (2) |
| GSE3651 |
The AIN-centered DCN of delayed-type eyeblink conditioned mice: 3-d paired training and sham negative control groups |
| GSE3713 |
The AIN-centered DCN of delayed-type eyeblink conditioned mice |
|
