|
| Status |
Public on Jul 01, 2006 |
| Title |
Ngn3 EB3 ON replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
tetracycline inducible Ngn3 ESC differentiated for 3 days as EB with doxycycline
|
| Organism |
Mus musculus |
| Characteristics |
tetracycline inducible Ngn3 ESC differentiated for 3 days as EB with doxycycline
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol extraction, RNeasy column purification
|
| Label |
biotin
|
| Label protocol |
10ug total RNA
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
16 embryonic stem cell (ESC) samples (8 groups in duplicate) were processed for microarray analysis using the Affymetrix Mouse Genome 430 2.0 GeneChip. The samples included the parental Ainv15 ESC line (1) undifferentiated (Ainv15 ESC), (2) differentiated for 3 days as embryoid bodies (Ainv15 EB3) or (3) for 10 days as embryoid bodies (Ainv15 EB10). The remaining samples included tetracycline inducible Ngn3 ESC line derived from the parental Ainv15 ESC line (4) after differentiation and addition of doxycycline for 3 days without embryoid body formation (Ngn3 ES3 ON), (5 and 6) after differentiation as embryoid bodies for 3 days with (Ngn3 EB3 ON) or without (Ngn3 EB3 OFF) doxycycline, and (7 and 8) after differentiation as embryoid bodies for 10 days with (Ngn3 EB10 ON) or without (Ngn3 EB10 OFF) doxycycline.
|
| Data processing |
Stratagene ArrayAssist GCRMA Normalization
|
| |
|
| Submission date |
Nov 23, 2005 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Nathan R Treff |
| E-mail(s) |
NTreff@rmanj.com
|
| Phone |
973-871-1236
|
| Organization name |
RMA of NJ
|
| Department |
Molecular Biology
|
| Lab |
Suite 100
|
| Street address |
111 Madison Ave
|
| City |
Morristown |
| State/province |
NJ |
| ZIP/Postal code |
07960 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE3653 |
Inducible Ngn3 Embryonic Stem Cells |
|
| Relations |
| Reanalyzed by |
GSE119085 |
