|
| Status |
Public on Dec 20, 2011 |
| Title |
DSS + MTA rep5 |
| Sample type |
RNA |
| |
|
| Source name |
whole colon
|
| Organism |
Mus musculus |
| Characteristics |
treatment: Dextran Sulfate Sodium + Methylthioadenosine
strain: C57Bl/6J
gender: Male
tissue: colon
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Qiagen's Rneasy Mini kit and the protocol with extra washes. Quantity and quality were verified using Nanodrop
|
| Label |
Cy3
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
Cubic Spline using Illumina Beadchip Studio
|
| |
|
| Submission date |
Dec 19, 2011 |
| Last update date |
Dec 20, 2011 |
| Contact name |
Nancy Benight |
| E-mail(s) |
dburrin@bcm.edu
|
| Organization name |
Baylor College of Medicine
|
| Street address |
1100 Bates Street
|
| City |
Houston |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE34553 |
Gene Array Analysis of Methylthioadenosine Supplementation in Dextran Sulfate Sodium Induced Colitis |
|
