|
| Status |
Public on Jul 01, 2012 |
| Title |
vector-transfected RAW264.7 mock 2 |
| Sample type |
RNA |
| |
|
| Source name |
vector-transfected RAW264.7 mock
|
| Organism |
Mus musculus |
| Characteristics |
cloneid: 3
cell line: RAW264.7
transfection: vector
|
| Treatment protocol |
Ms4a8a-transfected and empty vector-transfected RAW264.7 cells were incubated for 6 h with 5 µg/ml LPS in DMEM medium supplemented with 10% FCS, 100 IU penicillin, 100 µg/ml streptomycin, 1% pyruvic acid, 1% non essential amino acids.
|
| Growth protocol |
Raw 264.7 cells were cultivated with DMEM medium supplemented with 10%FCS, 100IU penicillin, 100 µg/ml penicillin, 100 µg/ml streptomycin, 1% pyruvic acid, 1% non essential amino acids. Authentication of cell lines was assured by regular morphology checks and growth curve analyses. Cells were regularly tested for mycoplasma infection by PCR.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from these cultures using Qiagen RNeasy Kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
Gene expression from empty vector transfected Raw264.7 macrophages
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 4, from SAS. Gene expression profiling was performed using arrays of Mouse430_2 -type from Affymetrix. A Custom CDF Version 14 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
|
| |
|
| Submission date |
Dec 19, 2011 |
| Last update date |
Jul 01, 2012 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL15041 |
| Series (1) |
| GSE34563 |
Gene expression data of Ms4a8a transfected Raw 264.7 macrophage-like cells |
|
