|
| Status |
Public on Mar 27, 2013 |
| Title |
murine atherosclerotic plaque on 3 month high fat diet Replicate 3, b |
| Sample type |
RNA |
| |
|
| Source name |
murine atherosclerotic plaque on 3 month high fat diet
|
| Organism |
Mus musculus |
| Characteristics |
strain: apoe-/-
age: 6-8 weeks
Sex: Female
tissue: aortic root
|
| Treatment protocol |
mice were fed on 3 month high fat diet
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RecoverAll total nucleic acid isolation kit (Applied Biosystems) see http://www3.appliedbiosystems.com/cms/groups/mcb_support/documents/generaldocuments/cms_056616.pdf
|
| Label |
FAM
|
| Label protocol |
N/A
|
| |
|
| Hybridization protocol |
N/A
|
| Scan protocol |
N/A
|
| Description |
Biological replicate 3 of 4. early atherosclerotic plaque
|
| Data processing |
Taqman low density arrays were run on the 7900 HT Sequence Detection system. The ABI TaqMan SDS v2.3 software was utilized to obtain raw CT values. To review results, the raw CT data (SDS file format) were exported from the Plate Centric View into the ABI TaqMan RQ manager software. Automatic baseline and manual CTs were calculated with the RQ Manager Software for all samples. Statistical analysis: The global data set of miRs from card A and B was used for analysis. Data were calculated using Real Time StatMiner Tools [Integromics. http://www.integromics.com/StatMiner.php]. Input data for analysis consisted of all samples. CT values were normalized to the most stable endogenous controls (snoRNA202, snoRNA135, snoRNA429 and MammU6 for Card A and B) according to the Genorm algorithm.
|
| |
|
| Submission date |
Dec 22, 2011 |
| Last update date |
Mar 27, 2013 |
| Contact name |
Andreas Schober |
| E-mail(s) |
aschober@med.lmu.de
|
| Organization name |
Ludwig-Maximilians-University
|
| Department |
Institute for Cardiovascular Prevention
|
| Street address |
Pettenkoferstr. 9
|
| City |
Munich |
| State/province |
Bavaria |
| ZIP/Postal code |
80336 |
| Country |
Germany |
| |
|
| Platform ID |
GPL15053 |
| Series (2) |
| GSE34647 |
MiRNA expression profile in murine advanced atherosclerotic plaque compared with early atherosclerotic plaque |
| GSE34648 |
MiRNA expression profile in murine |
|
