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Sample GSM8543 Query DataSets for GSM8543
Status Public on Dec 19, 2003
Title Hour 2 No Yeast (NY)
Sample type RNA
 
Source name Whole animal (Drosophila)
Organism Drosophila melanogaster
Extracted molecule total RNA
 
Description Hour 2 No Yeast Treatment (NY).

Larvae were reared on standard diet until early third instar, at which time they were washed and transferred to standard diet lacking yeast. The animals remained on this diet until four days after emergence, when one group of adults was switched back to standard diet containing yeast (group Y) while another remained on the diet lacking yeast (group NY). Flies from both groups were killed every hour for the next twelve hours, creating 24 samples across the two treatments. In addition, four samples of flies were killed just before the start of the time course and used as baseline replicates for the no yeast (NY) and yeast (Y) treatments. Baseline replicates were temporally ordered as noted for change-point analysis. No yeast (NY) treatment samples at hours four and eight did not yield microarray data due to insufficient RNA.

Total RNA was extracted from whole animals using Trizol (Invitrogen). Sample processing and microarray hybridization/scanning were performed at the Brown University Center for Genetics and Genomics according to Affymetrix protocol. Microarray data was normalized by DNA-Chip Analyzer (dChip, http://www.dchip.org), which utilizes an invariant difference selection (IDS) algorithm to construct a normalization relation.
Keywords = insulin, diet, nutrition
 
Submission date Jul 22, 2003
Last update date May 28, 2005
Contact name Boris Gershman
E-mail(s) Boris_Gershman@brown.edu
Phone 401- 863-3455
Organization name Brown University
Department Ecology and Evolutionary Biology
Lab Marc Tatar Lab
Street address Attn: Marc Tatar, Box G-W
City Providence
State/province RI
ZIP/Postal code 02912
Country USA
 
Platform ID GPL72
Series (2)
GSE547 No Yeast (NY) Treatment Series
GSE562 Yeast versus no yeast nutrition study

Data table header descriptions
ID_REF
VALUE Signal (transcript abundance) after dChip normalization
ABS_CALL Present (P), Marginal (M), or Absent (A) call provided by dChip normalization
STD ERR Standard error provided by dChip normalization

Data table
ID_REF VALUE ABS_CALL STD ERR
AFFX-MurIL2_at 102.302 A 3.42247
AFFX-MurIL10_at 50.741 A 1.48687
AFFX-MurIL4_at 39.4999 A 1.58373
AFFX-MurFAS_at 35.3373 A 1.66948
AFFX-BioB-5_at 239.069 P 5.95973
AFFX-BioB-M_at 255.179 P 6.06581
AFFX-BioB-3_at 376.303 P 8.92799
AFFX-BioC-5_at 371.019 P 7.71656
AFFX-BioC-3_at 699.25 P 8.52007
AFFX-BioDn-5_at 434.59 P 4.34962
AFFX-BioDn-3_at 2975.19 P 101.018
AFFX-CreX-5_at 4652.74 P 85.4845
AFFX-CreX-3_at 7247.21 P 143.17
AFFX-DapX-5_at 37.4382 P 1.4578
AFFX-DapX-M_at 103.797 A 3.10484
AFFX-DapX-3_at 50.9023 A 3.04834
AFFX-LysX-5_at 17.7985 A 1.11602
AFFX-LysX-M_at 82.3901 A 1.99033
AFFX-LysX-3_at 53.2272 P 2.2156
AFFX-PheX-5_at 39.3581 A 2.34396

Total number of rows: 14010

Table truncated, full table size 381 Kbytes.




Supplementary data files not provided

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