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Sample GSM854300 Query DataSets for GSM854300
Status Public on Dec 28, 2011
Title DC.pDC.8+.SLN#2
Sample type RNA
 
Source name Skin LN
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: PI SA-PECy7
genetic background: C57BL/6J
phenotype markers: B220-FITC CD45-APC CD11c-eFluor780 GR1-PE CD8-eFluor450
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol gentle teasing using forceps, Liberase DL + DNase I digestion 15 min at 37C depletion of NK1.1, CD3e, CD19 and TER119 positive cells using MACS system
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description DC.pDC.8+.SLN#2, Skin lymph node CD8+ plasmacytoid dendritic cells CD11cint/CD8a+/CD4+/B220+/GR1+
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Dec 27, 2011
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1
Relations
Reanalyzed by GSE74316

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 17.659
10344622 240.8892
10344624 398.3684
10344633 729.8468
10344637 274.8059
10344653 16.5644
10344658 220.7382
10344674 14.3232
10344679 36.0611
10344707 358.9627
10344713 205.5478
10344723 169.3768
10344725 38.3314
10344741 2464.5588
10344743 20.2302
10344750 828.0888
10344772 30.7716
10344789 191.7576
10344797 304.461
10344799 240.842

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM854300_EA07068_130455_MOGENE-1_0-ST-V1_DC.PDC.8+.SLN_2.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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