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Status
Public on Dec 28, 2011
Title
DC.pDC.8+.SLN#2
Sample type
RNA
Source name
Skin LN
Organism
Mus musculus
Characteristics
age: 6 weeks exclusion markers: PI SA-PECy7 genetic background: C57BL/6J phenotype markers: B220-FITC CD45-APC CD11c-eFluor780 GR1-PE CD8-eFluor450 separation method: dissection/trimming Sex: male
Extracted molecule
total RNA
Extraction protocol
gentle teasing using forceps, Liberase DL + DNase I digestion 15 min at 37C depletion of NK1.1, CD3e, CD19 and TER119 positive cells using MACS system
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
DC.pDC.8+.SLN#2, Skin lymph node CD8+ plasmacytoid dendritic cells CD11cint/CD8a+/CD4+/B220+/GR1+
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Dec 27, 2011
Last update date
May 24, 2016
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246
Series (1)
Relations
Reanalyzed by
GSE74316
Data table header descriptions
ID_REF
VALUE
RMA normalized signal intensity
Data table
ID_REF
VALUE
10344620
17.659
10344622
240.8892
10344624
398.3684
10344633
729.8468
10344637
274.8059
10344653
16.5644
10344658
220.7382
10344674
14.3232
10344679
36.0611
10344707
358.9627
10344713
205.5478
10344723
169.3768
10344725
38.3314
10344741
2464.5588
10344743
20.2302
10344750
828.0888
10344772
30.7716
10344789
191.7576
10344797
304.461
10344799
240.842
Total number of rows: 24922 Table truncated, full table size 422 Kbytes .Supplementary file
Size
Download
File type/resource
GSM854300_EA07068_130455_MOGENE-1_0-ST-V1_DC.PDC.8+.SLN_2.CEL.gz
4.3 Mb
(ftp) (http)
CEL
Processed data included within Sample table