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Sample GSM854994 Query DataSets for GSM854994
Status Public on Feb 04, 2012
Title CR diet FLOX 2
Sample type RNA
 
Source name TA/EDL muscle, CR diet, FLOX genotype
Organism Mus musculus
Characteristics tissue: TA/EDL muscles
diet: CR diet
genotype: FLOX
Treatment protocol Mice were fed AIN-93M diet (BioServe, NJ) ad libitum for at least 10 days until baseline food intake was determined. Mice were randomly divided into control (C) or calorie restricted (CR) groups. Control mice were fed 90% and CR mice were fed 80% of their ad libitum food intake. After one week, CR mice were switched to AIN-93M 40% CR diet and fed 60% of their ad libitum intake until sacrifice approximately 11 weeks later.
Growth protocol Individually-housed littermate FLOX and MKO mice were used for all experiments.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to manufacturer instructions (Invitrogen). RNA was cleaned using RNeasy MiniKit from Qiagen.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip mouse Genome Array Mouse430_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Data processing Raw data were processed using the dChip algorithm in GenePattern. All further data analysis was performed using GenePattern modules.
 
Submission date Dec 29, 2011
Last update date Feb 04, 2012
Contact name Marcia C Haigis
Organization name Harvard Medical School
Street address 77 Ave Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL1261
Series (1)
GSE34773 Skeletal muscle PGC-1a mediates mitochondrial, but not metabolic, changes during calorie restriction.

Data table header descriptions
ID_REF
VALUE dChip

Data table
ID_REF VALUE
1415670_at 655.1212956
1415671_at 982.9753886
1415672_at 1750.409263
1415673_at 611.5697208
1415674_a_at 539.7353511
1415675_at 383.5715314
1415676_a_at 5039.72877
1415677_at 1359.957083
1415678_at 3584.538758
1415679_at 3017.414553
1415680_at 979.0290856
1415681_at 1406.259797
1415682_at 570.1106253
1415683_at 1382.756219
1415684_at 626.2111134
1415685_at 544.2788596
1415686_at 1159.058509
1415687_a_at 5735.031243
1415688_at 3899.954915
1415689_s_at 538.582078

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM854994_15-cr-flox.CEL.gz 3.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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