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Sample GSM854995 Query DataSets for GSM854995
Status Public on Feb 04, 2012
Title CR diet FLOX 3
Sample type RNA
 
Source name TA/EDL muscle, CR diet, FLOX genotype
Organism Mus musculus
Characteristics tissue: TA/EDL muscles
diet: CR diet
genotype: FLOX
Treatment protocol Mice were fed AIN-93M diet (BioServe, NJ) ad libitum for at least 10 days until baseline food intake was determined. Mice were randomly divided into control (C) or calorie restricted (CR) groups. Control mice were fed 90% and CR mice were fed 80% of their ad libitum food intake. After one week, CR mice were switched to AIN-93M 40% CR diet and fed 60% of their ad libitum intake until sacrifice approximately 11 weeks later.
Growth protocol Individually-housed littermate FLOX and MKO mice were used for all experiments.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to manufacturer instructions (Invitrogen). RNA was cleaned using RNeasy MiniKit from Qiagen.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip mouse Genome Array Mouse430_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Data processing Raw data were processed using the dChip algorithm in GenePattern. All further data analysis was performed using GenePattern modules.
 
Submission date Dec 29, 2011
Last update date Feb 04, 2012
Contact name Marcia C Haigis
Organization name Harvard Medical School
Street address 77 Ave Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL1261
Series (1)
GSE34773 Skeletal muscle PGC-1a mediates mitochondrial, but not metabolic, changes during calorie restriction.

Data table header descriptions
ID_REF
VALUE dChip

Data table
ID_REF VALUE
1415670_at 670.9597022
1415671_at 1064.938565
1415672_at 1776.358201
1415673_at 594.2355448
1415674_a_at 555.7785087
1415675_at 383.258044
1415676_a_at 4867.835151
1415677_at 1215.654724
1415678_at 3874.958735
1415679_at 3015.832858
1415680_at 858.7161241
1415681_at 1374.919692
1415682_at 606.8914892
1415683_at 1389.390157
1415684_at 639.1096734
1415685_at 526.2147853
1415686_at 1347.246162
1415687_a_at 6308.013502
1415688_at 4036.38648
1415689_s_at 505.1158913

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM854995_16-cr-flox.CEL.gz 3.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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