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Sample GSM854997 Query DataSets for GSM854997
Status Public on Feb 04, 2012
Title CR diet FLOX 5
Sample type RNA
 
Source name TA/EDL muscle, CR diet, FLOX genotype
Organism Mus musculus
Characteristics tissue: TA/EDL muscles
diet: CR diet
genotype: FLOX
Treatment protocol Mice were fed AIN-93M diet (BioServe, NJ) ad libitum for at least 10 days until baseline food intake was determined. Mice were randomly divided into control (C) or calorie restricted (CR) groups. Control mice were fed 90% and CR mice were fed 80% of their ad libitum food intake. After one week, CR mice were switched to AIN-93M 40% CR diet and fed 60% of their ad libitum intake until sacrifice approximately 11 weeks later.
Growth protocol Individually-housed littermate FLOX and MKO mice were used for all experiments.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to manufacturer instructions (Invitrogen). RNA was cleaned using RNeasy MiniKit from Qiagen.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip mouse Genome Array Mouse430_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Data processing Raw data were processed using the dChip algorithm in GenePattern. All further data analysis was performed using GenePattern modules.
 
Submission date Dec 29, 2011
Last update date Feb 04, 2012
Contact name Marcia C Haigis
Organization name Harvard Medical School
Street address 77 Ave Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL1261
Series (1)
GSE34773 Skeletal muscle PGC-1a mediates mitochondrial, but not metabolic, changes during calorie restriction.

Data table header descriptions
ID_REF
VALUE dChip

Data table
ID_REF VALUE
1415670_at 761.431852
1415671_at 1114.002435
1415672_at 1797.520824
1415673_at 641.465533
1415674_a_at 506.2997353
1415675_at 393.8907918
1415676_a_at 4720.079805
1415677_at 1105.809654
1415678_at 3945.652136
1415679_at 3101.954799
1415680_at 928.0568985
1415681_at 1452.889543
1415682_at 602.9017879
1415683_at 1461.882875
1415684_at 629.9857931
1415685_at 709.4579822
1415686_at 1287.164929
1415687_a_at 5853.808293
1415688_at 4128.728038
1415689_s_at 516.6550845

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM854997_18-cr-flox.CEL.gz 3.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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