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Sample GSM854999 Query DataSets for GSM854999
Status Public on Feb 04, 2012
Title CR diet MKO 1
Sample type RNA
 
Source name TA/EDL muscle, CR diet, MKO genotype
Organism Mus musculus
Characteristics tissue: TA/EDL muscles
diet: CR diet
genotype: MKO
Treatment protocol Mice were fed AIN-93M diet (BioServe, NJ) ad libitum for at least 10 days until baseline food intake was determined. Mice were randomly divided into control (C) or calorie restricted (CR) groups. Control mice were fed 90% and CR mice were fed 80% of their ad libitum food intake. After one week, CR mice were switched to AIN-93M 40% CR diet and fed 60% of their ad libitum intake until sacrifice approximately 11 weeks later.
Growth protocol Individually-housed littermate FLOX and MKO mice were used for all experiments.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to manufacturer instructions (Invitrogen). RNA was cleaned using RNeasy MiniKit from Qiagen.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip mouse Genome Array Mouse430_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Data processing Raw data were processed using the dChip algorithm in GenePattern. All further data analysis was performed using GenePattern modules.
 
Submission date Dec 29, 2011
Last update date Feb 04, 2012
Contact name Marcia C Haigis
Organization name Harvard Medical School
Street address 77 Ave Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL1261
Series (1)
GSE34773 Skeletal muscle PGC-1a mediates mitochondrial, but not metabolic, changes during calorie restriction.

Data table header descriptions
ID_REF
VALUE dChip

Data table
ID_REF VALUE
1415670_at 772.2847235
1415671_at 1167.344898
1415672_at 1978.981887
1415673_at 647.1158649
1415674_a_at 575.695596
1415675_at 407.698405
1415676_a_at 4774.225287
1415677_at 1140.199244
1415678_at 3848.139985
1415679_at 3370.9898
1415680_at 947.1652383
1415681_at 1233.601277
1415682_at 637.5006937
1415683_at 1365.420996
1415684_at 640.3831049
1415685_at 522.34652
1415686_at 1351.602347
1415687_a_at 5014.132097
1415688_at 4494.490428
1415689_s_at 501.1447236

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM854999_20-cr-mko.CEL.gz 3.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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