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Sample GSM855000 Query DataSets for GSM855000
Status Public on Feb 04, 2012
Title CR diet MKO 2
Sample type RNA
 
Source name TA/EDL muscle, CR diet, MKO genotype
Organism Mus musculus
Characteristics tissue: TA/EDL muscles
diet: CR diet
genotype: MKO
Treatment protocol Mice were fed AIN-93M diet (BioServe, NJ) ad libitum for at least 10 days until baseline food intake was determined. Mice were randomly divided into control (C) or calorie restricted (CR) groups. Control mice were fed 90% and CR mice were fed 80% of their ad libitum food intake. After one week, CR mice were switched to AIN-93M 40% CR diet and fed 60% of their ad libitum intake until sacrifice approximately 11 weeks later.
Growth protocol Individually-housed littermate FLOX and MKO mice were used for all experiments.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to manufacturer instructions (Invitrogen). RNA was cleaned using RNeasy MiniKit from Qiagen.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip mouse Genome Array Mouse430_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Data processing Raw data were processed using the dChip algorithm in GenePattern. All further data analysis was performed using GenePattern modules.
 
Submission date Dec 29, 2011
Last update date Feb 04, 2012
Contact name Marcia C Haigis
Organization name Harvard Medical School
Street address 77 Ave Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL1261
Series (1)
GSE34773 Skeletal muscle PGC-1a mediates mitochondrial, but not metabolic, changes during calorie restriction.

Data table header descriptions
ID_REF
VALUE dChip

Data table
ID_REF VALUE
1415670_at 772.0456504
1415671_at 1109.117727
1415672_at 1904.493171
1415673_at 643.3339728
1415674_a_at 537.0513494
1415675_at 388.5827386
1415676_a_at 4828.464282
1415677_at 919.6988292
1415678_at 4272.748189
1415679_at 3305.315944
1415680_at 859.3247598
1415681_at 1309.787733
1415682_at 639.6308002
1415683_at 1384.785099
1415684_at 632.9568047
1415685_at 583.5275782
1415686_at 1589.043897
1415687_a_at 5760.081314
1415688_at 4345.876788
1415689_s_at 479.2456327

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM855000_21-cr-mko.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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