|
| Status |
Public on Dec 31, 2011 |
| Title |
oocyte from 12day HDAC1 conditional knocked-out mouse, biological rep4 |
| Sample type |
RNA |
| |
|
| Source name |
oocyte from 12day HDAC1 conditional knocked-out
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
genotype/variation: HDAC1 conditional knocked-out
age: 12day-old
tissue source: ovary
tissue: oocyte
|
| Treatment protocol |
To establish the in vivo role(s) of HDAC1 and HDAC2 during oocyte growth, we generated conditional mutant oocytes using Zp3-Cre transgenic mice (PMID 9016703, 12912906) in combination with conditional loss of function alleles of Hdac1 or/and Hdac2
|
| Growth protocol |
from 12 day old mouse ovary
|
| Extracted molecule |
total RNA |
| Extraction protocol |
using the PicoPure RNA kit (Arcturus) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
FL-OvationTM cDNA Biotin Module V2 (NuGen)
|
| |
|
| Hybridization protocol |
Following fragmentation, Four independent biological replicates were hybridized to Affymetrix GeneChip Mouse 1.1 ST microarrays
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from oocyte from 12day mouse
|
| Data processing |
Raw microarray data were analyzed as previously described using MAS5, GeneSpring v7, SAM and EASE software
|
| |
|
| Submission date |
Dec 29, 2011 |
| Last update date |
Jan 02, 2012 |
| Contact name |
Hua Pan |
| E-mail(s) |
huapan@sas.upenn.edu
|
| Organization name |
University of Pennsylvania
|
| Department |
Department of Biology
|
| Street address |
415 S University Avenue
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL11533 |
| Series (1) |
| GSE34786 |
Expression data from HDAC1 and HDAC2 knocked-out mouse oocyte |
|
