|
| Status |
Public on May 22, 2012 |
| Title |
Epiblast stem cells |
| Sample type |
RNA |
| |
|
| Source name |
epiblast stem cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: ICR
gender: male
cell type: E5.5 mouse epiblast
passage: 16
embryo stage: late post-implantation epiblast
|
| Growth protocol |
EpiSC were cultured in N2B27 media (Ying and Smith, 2003) supplemented with 10 ng/ml Activin A and 12 ng/ml bFGF on fibronectin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction protocol and RNeasy columns.
|
| Label |
biotin
|
| Label protocol |
As per Affymetrix protocol.
|
| |
|
| Hybridization protocol |
As per Affymetrix protocol.
|
| Scan protocol |
As per Affymetrix protocol.
|
| Description |
Gene expression of mouse epiblast stem cells.
|
| Data processing |
Used the limma package in R. Applied RMA.
|
| |
|
| Submission date |
Jan 01, 2012 |
| Last update date |
May 23, 2012 |
| Contact name |
Brian Joseph Cox |
| E-mail(s) |
b.cox@utoronto.ca
|
| Organization name |
University of Toronto
|
| Department |
Physiology
|
| Lab |
Cox System Biology
|
| Street address |
1 King's College Circle, Rm 3360
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5S1A8 |
| Country |
Canada |
| |
|
| Platform ID |
GPL11533 |
| Series (1) |
| GSE34799 |
Stem cell lines of the early mouse embryo |
|
