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Status |
Public on Aug 08, 2012 |
Title |
NIH-3T3_miR-378mimic-transfected_48h_rep3 |
Sample type |
RNA |
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Source name |
NIH-3T3_miR-378mimic-transfected_48h
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Organism |
Mus musculus |
Characteristics |
cell line: NIH-3T3 cell type: murine fibroblast cells transfectant: miR-378 mimic
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Treatment protocol |
Cells were transfected with 25nM RNA complexed to DharmaFECT 1 and analysed after 48 hours.
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Growth protocol |
NIH-3T3 fibroblasts were grown in RPMI supplemented with 10% FCS, 2mM L-glutamine, 0.25U/ml penicillin and 100U/ml streptomycin at 37C 5%CO2.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the Qiagen miRNeasy kit as described by the manufacturer.
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Label |
biotin
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Label protocol |
Total RNA was converted to cDNA using the GeneChip WT cDNA Synthesis and Amplification Kit (Affymetrix) and biotinylated using the GeneChip® WT Terminal Labeling Kit (Affymetrix).
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Hybridization protocol |
Following fragmentation, 5.5 ug of sscDNA were hybridized for 16 hr at 45C on Affymetrix GeneChip ® Mouse Gene 1.0 ST Array.
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Scan protocol |
GeneChips were scanned using the Agilent Technologies Micro Array Scanner #G2505B.
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Description |
miR-378 m_3 gene expression data from cells transfected with a mmu-miR-378-3p mimic.
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Data processing |
The data were analyzed with Partek Genomics Suite version 6.5 using Affymetrix default analysis settings and RMA as normalization method. Based on the log 2 value of negative control probes of each chip, the background fluorescence was calculated in StatPlus and used to eliminate the microarray's background noise.
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Submission date |
Jan 05, 2012 |
Last update date |
Aug 09, 2012 |
Contact name |
Dominik Andreas Rückerl |
E-mail(s) |
dominik.ruckerl@manchester.ac.uk
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Organization name |
The University of Manchester
|
Department |
Faculty of Biology, Medicine and Health
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Street address |
Oxford Road
|
City |
Manchester |
ZIP/Postal code |
M13 9PT |
Country |
United Kingdom |
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Platform ID |
GPL6246 |
Series (1) |
GSE34873 |
Experimental identification of miR-378-3p targets by overexpression and silencing in murine NIH-3T3 fibroblasts |
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